Method for preparing lactic acid bacteria feed additive by using eleocharis tuberosa starch wastewater

Abstract

The invention discloses a method for preparing lactic acid bacteria feed additive by using eleocharis tuberosa starch wastewater. The method is characterized by adopting the direct lactic acid bacteria agent of which the strain is lactobacillus plantarum as the initial strain, and comprising the following step of: carrying out the two-stage amplification, wherein the amplification condition is to seal at the natural environment temperature and stay the night, the rate of the first-stage volume to the amplification proportion is 1:5000-10000, and the rate of the second-stage volume to the amplification proportion is 1:4-9; performing in-series continuous fermentation; and obtaining the lactic acid bacteria feed additive after fermenting for 10 hours. According to the invention, the raw material is easily available, heat treatment during the production is not needed, and sterilization and preservation operations are not needed, so that a large amount of energy can be saved, the cost can be reduced, discharge of wastewater is avoided, the process is environmental-friendly, and the industrial production can be smoothly achieved.

Description

technical field [0001] The invention belongs to the field of feed additives, in particular to a method for producing lactic acid bacteria feed additives by utilizing horseshoe starch wastewater. Background technique [0002] Lactic acid bacteria can promote animal growth, improve feed digestibility and biological potency, inhibit the growth of spoilage bacteria in the intestine, ensure the health of animals, reduce or even replace the use of antibiotics, etc., and are increasingly used in livestock, poultry and aquatic products wider. my country's Ministry of Agriculture stipulates that there are 16 kinds of microorganisms that can be used in feed additives, 11 of which are lactic acid bacteria. The nutritional requirements of lactic acid bacteria are extremely complex. So far, the preparation of lactic acid bacteria reported so far is almost all carried out by fermentation using a medium synthesized from glucose, peptone, yeast extract, inorganic salts, trace elements, vit...

AI Technical Summary

Problems solved by technology

The nutritional requirements of lactic acid bacteria are extremely complex. So far, the preparation of lactic acid bacteria reported so far is almost all carried out by fermentation using a medium synthesized from glucose, peptone, yeast extract, inorganic salts, trace elements, vitamins, etc., such as the Chinese patent " Preparation method of lactic acid bacteria biological feed additive" (patent number: 201010122147), "a high-density fermentation medium and fermentation method of lactic acid bacteria for feeding" (patent number 201110116555), Chinese patent application "a high-density culture method of lactic acid bacteria" (Application No. 200710004941.2.), "A High Density Cultivation Method for Lactic Acid Bacteria" (Application No. 200910057650), and "Development of High-Efficiency Lactic Acid Bacteria Direct-Injected Starter" Tan Huan. [J]. Hunan Agricultural Science, 2012, (9) : 102-104, 107 and other papers have all used the above method, and the lactic acid bacteria count in the liquid after cultivation can reach 10 9 More than cfu / ml, but the cost of raw materials is high, and the cost of raw materials accounts for 30%-50% of the fermentation cost; and the medium must be sterilized throughout the process, and the temperature must be kept at 36°C or 42°C for cultivation, and the energy consumption is also low. High; in addition, the treatment of fermentation waste liquid is also very complicated

Occasionally, there are reports of using biomass raw materials such as pineapple peel, oyster mushroom dipping juice and potato juice to enrich bacteria, "Research on New Type Lactic Acid Bacteria Enrichment Medium", Du Lei et al. [J] Dairy Science and Technology, .2012,35(1 )4-7.) In addition to the disadvantages of high energy consumption such as sterilization and constant temperature, this method also has the disadvantages of unstable source of raw materials and difficult preservation

Therefore, the industrialized production of lactic acid bacteria has not yet been realized in my country.