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Genetic recombinant human-like collagen

A technology of human collagen and human-derived collagen, applied in the field of genetic engineering, can solve the problems of insufficient purity, inability to meet higher requirements for purity, impurity removal of impurity proteins and partially degraded proteins, etc.

Active Publication Date: 2013-05-15
JIANGSU TRAUTEC MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its purification method only uses traditional non-specific macromolecular separation techniques such as ultrafiltration, gel filtration, and ion exchange. The purity is not high enough, and impurities and partially degraded proteins cannot be completely removed. Higher requirements for purity

Method used

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  • Genetic recombinant human-like collagen
  • Genetic recombinant human-like collagen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The recombinant human collagen has a total length of 474 amino acids, which is composed of two identical human type III collagen fragments connected in series, with 6 histidine residues connected at the end. Its sequence (SEQ ID NO.1) is as follows:

[0069] AGNTGAPGSP GVSGPKGDAG QPGEKGSPGA QGPPGAPGPL GIAGITGARG 50 LAGPPGMPGP RGSPGPQGVK GESGKPGANG LSGERGPPGP QGLPGLAGTA 100 GEPGRDGNPG SDGLPGRDGS PGGKGDRGEN GSPGAPGAPG HPGPPGPVGP 150 AGKSGDRGES GPAGPAGAPG PAGSRGAPGP QGPRGDKGET GERGAAGIKG 200 HRGFPGNPGA PGSPGPAGQQ GAIGSPGPAE FTAGNTGAPG SPGVSGPKGD 250 AGQPGEKGSP GAQGPPGAPG PLGIAGITGA RGLAGPPGMP GPRGSPGPQG 300

[0070] VKGESGKPGA NGLSGERGPP GPQGLPGLAG TAGEPGRDGN PGSDGLPGRD 350 GSPGGKGDRG ENGSPGAPGA PGHGPGPGPV GPAGKSGDRG ESGPAGPAGA 400 PGPAGSRGAP GPQGPRGDKG ETGERGAAGI KGHRGFPGNP GAPGSPGPAG 450 QQHQGAIGSPGP4 ADGH

[0071] The protein has a single-chain structure, in which 1-229 and 233-461 are the same type III collagen peptides, and there is an EFT connection between th...

Embodiment 2

[0241] Now provide the embodiment that genetically recombinant human collagen is used for cosmetic and skin care products for external use:

[0242] According to the following mass ratio, dissolve each raw material with water, and stir well to form a colorless, odorless and transparent moisturizing skin care essence:

[0243] Gene recombinant human collagen 1%

[0244] Sodium Hyaluronate 0.1%

[0245] Beta-Glucan 1%

[0246] Jojoba Esters 0.5%

[0247] Serine 0.1%

[0248] Sorbitol 5%

[0249] Betaine 5%

[0250] Sodium Lactate 3%

[0251] How to use: After cleansing in the morning and evening, apply directly to the face, gently pat until fully absorbed.

Embodiment 3

[0253] According to the following mass ratio, dissolve each raw material with water, and stir well to form a colorless, odorless and transparent moisturizing skin care essence:

[0254] Gene recombinant human collagen 0.01%

[0255] Sodium Hyaluronate 0.01%

[0256] Beta-glucan 0.01%

[0257] Jojoba Esters 0.01%

[0258] Serine 0.01%

[0259] Sorbitol 1%

[0260] Betaine 1%

[0261] Sodium Lactate 0.1%

[0262] How to use: After cleansing in the morning and evening, apply directly to the face, gently pat until fully absorbed.

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Abstract

The invention discloses humanized genetic recombinant human-like collagen produced by eukaryotic bacteria. The humanized genetic recombinant human-like collagen has amino acid with the total length of 474, and is formed by connecting two sections of completely-identical human III-type collagen sections in series; the end C is connected with six histidine residue groups serving as specificity affinity purification markers. The performance of the genetic recombination human-like collagen is superior to animal collagens and original nuclear engineering bacteria recombinant collagens; and with the specificity affinity purification markers, the high-purity product is easily acquired.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a recombinant collagen and a production method thereof. Background technique [0002] Collagen is a biopolymer substance that maintains the shape and structure of skin and tissues and organs, and is also an important substance for repairing various damaged tissues. It mainly exists in the skin, bone, cartilage, teeth, tendons, ligaments and blood vessels of humans and animals. It is an important structural protein in connective tissue and plays the role of supporting organs and protecting the body. Collagen plays an important role in maintaining the normal physiological functions of cells, tissues and organs and repairing damage. Collagen can give the necessary nutrients to the skin layer containing collagen, strengthen the activity of collagen in the skin, maintain the moisture of the stratum corneum and the integrity of the fiber structure, improve the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/22C12N1/19C12N15/12C12N15/81C12P21/02A61K8/92A61K8/65A61Q19/00A61L27/24C12R1/84
Inventor 梁亮
Owner JIANGSU TRAUTEC MEDICAL TECH CO LTD
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