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Method for extracting taxusflavone from taxus chinensis leaves

A technology of southern yew and taxus flavonoids, which is applied in the field of taxane flavonoids extraction, can solve the problems of low purity and complex extraction of taxus flavonoids, and achieve the effect of convenient implementation, simple extraction method and simple method

Active Publication Date: 2013-05-15
宁波泰康红豆杉生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This extraction method is complicated to extract, and the purity of taxoflavone is lower, not more than 70%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] A method for extracting taxus flavonoids from southern yew leaves. First, fresh southern yew leaves are dried at 50°C for 24 hours, pulverized, passed through a No. 8 sieve into powder, weighed 50g, and packed into a super In the critical extraction tank; in the supercritical extraction tank, the extraction temperature is 28 ° C, CO 2 The fluid pressure is 25MPa, the entrainer ethanol flow rate is 0.27 ml / min, and the extract is extracted for 1.5h; 25ml of methanol is added to the extract to absorb it as an absorption liquid, and the absorption liquid is chromatographed with AB-8 resin (commercially available), AB- 8 The resin is of weak polarity, with a surface area of ​​480-520 ( m 2 / g ), the particle radius is 13~14nm, and the water content is 64%; after chromatography, it is eluted with ethanol with a mass percentage concentration of 80%, to obtain the first eluent, and the first eluent is concentrated under reduced pressure at 500Pa for 60min to obtain The first ...

Embodiment 2

[0014] Basically the same as Example 1, the only difference is that the extraction temperature is 20 ° C, CO 2 The fluid pressure is 20MPa, the entrainer ethanol flow rate is 0.10 ml / min, and the extraction time is 3h. After polyamide chromatography, it is eluted with 20% ethanol by mass percentage, and the weight content of taxoflavone in the obtained powder is 75%.

Embodiment 3

[0016] Basically the same as Example 1, the only difference is that the extraction temperature is 40 ° C, CO 2 The fluid pressure is 40MPa, the entrainer ethanol flow rate is 0.40 ml / min, and the extraction time is 1h. After polyamide chromatography, it is eluted with 40% ethanol by mass percentage, and the weight content of taxoflavone in the obtained powder is 78%.

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Abstract

The invention discloses a method for extracting taxusflavone from taxus chinensis leaves, which comprises the following steps of: extracting with a supercritical extraction kettle; performing methanol absorption and AB-8 resin chromatography; performing 100-mesh polyamide chromatography and ethanol elution; and concentrating and drying the elution liquid to obtain light yellow powder containing taxusflavone, wherein the content of taxusflavone is 75-80% by weight, and the purity of the taxusflavone is relatively high; and the extraction method is simple, saves the step of chloroform degreasing, and thus the method for extracting taxusflavone from taxus chinensis leaves,realizes relatively high purity of taxusflavone and is simple and convenient to implement.

Description

technical field [0001] The invention relates to the extraction technology of taxoflavones, in particular to a method for extracting taxoflavones from southern yew leaves. Background technique [0002] Taxus leaves contain medicinal ingredients such as paclitaxel, taxoflavones and taxus polysaccharides, and taxoflavones contain a common mother nucleus C 6 -C 3 -C 6 It has the functions of regulating the cardiovascular system, anti-inflammatory and anti-immune effects, anti-microbial effects, effects on peptic ulcer and anti-tumor effects. The Chinese invention patent with the authorization number ZL200910157034.0 discloses an extract and extraction method of the leaves of Taxus chinensis. The extraction method includes drying the leaves, crushing and sieving, immersing in ethanol solvent, ultrasonic extraction under stirring, and concentrating the extract under reduced pressure. The step of removing alcohol, chloroform extraction and degreasing, and concentrating and dryi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/13A61P9/00A61P29/00A61P37/02A61P31/00A61P1/04A61P35/00A61K127/00
Inventor 王强谢雪娣
Owner 宁波泰康红豆杉生物工程有限公司
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