Preparation of ECL (electro chemical luminescence) DNA (Deoxyribose Nucleic Acid) sensor based on 3D paper chip, and application of sensor to simultaneous detection on Hg<2+> and Ag<+>

Abstract

The invention discloses preparation of an ECL (electro chemical luminescence) DNA (Deoxyribose Nucleic Acid) sensor based on a 3D paper chip, and application of the sensor to simultaneous detection on Hg<2+> and Ag<+>. The preparation method of the sensor (the schematic diagram of which is shown in the figure) comprises the following steps of: designing a micro-fluidic chip pattern on a computer, and preparing a 3D paper chip sensor; preparing nano materials such as a porous gold nanowire, PdAg alloy, a carbon point and P acid according to existing methods, preparing PdAg@CQDs and PdAg@P-acid with signal amplification functions as ECL probes, and respectively compounding the ECL probes with DNA subjected to specific binding with Hg<2+> and Ag<+>; and modifying the porous gold nanowire to the surface of an electrode of the sensor by an electrode surface modifying technology, and absorbing a DNA segment to prepare the DNA sensor. The method for simultaneously detecting Hg<2+> and Ag<+> comprises the following steps of: connecting the modified electrode to an ECL apparatus, and detecting Hg<2+> and Ag<+> in a sample extracting solution. The electrode of the sensor is strong in specificity and high in sensitivity, and is capable of reaching a p mol level. When the paper chip sensor easily processed is used for simultaneously detecting two materials on the same electrode, the detection efficiency is improved, and the cost is lowered.

Description

[0001] technical field [0002] The invention relates to the technical field of detection of trace heavy metal ions, more specifically to the preparation of a 3D paper chip-based electrochemiluminescence (ECL) DNA sensor capable of simultaneously detecting two kinds of heavy metal ions. The invention also relates to the use of the DNA The sensor detects the method of water sample, blood sample, and food. Background technique [0003] Heavy metals are those with a density greater than 4 or 5 g / cm -3 a class of metallic elements. With the rapid growth of population and the rapid development of modern industrial technology, many harmful substances enter the soil system, surface water systems such as rivers and oceans, and enter animals and plants through cyclic enrichment, which seriously affects human health. At present, environmental and health problems caused by heavy metal pollution have become one of the global concerns, and heavy metal pollution in my country is also ve...

AI Technical Summary

Problems solved by technology

At present, the methods for detecting heavy metal ions mainly include (1) atomic fluorescence spectrometry, which is characterized by high sensitivity and a large linear range, but the types of metals determined are limited; (2) inductively coupled plasma mass spectrometry, whose detection limit is higher than that of atomic The absorption rate is lower, but it is expensive and easy to be polluted; (3) High performance liquid chromatography, which can realize the simultaneous determination of multiple elements, but the choice of complexing agents is limited and has limitations; (4) Enzyme analysis (5) Immunoassay, which has high sensitivity and strong specificity, but the operation steps are complicated and the reagents expensive, relatively high cost

(6) Biose

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