Method for extracting flavonoids from Allium mongolicum Regel leaves
An extraction method and technology for flavonoids, which can be used in drug combinations, pharmaceutical formulations, plant raw materials, etc., and can solve problems such as less research.
Inactive Publication Date: 2013-05-01
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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Although there have been reports of flavonoids in Allium plants for a long time, there are few specific studies
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[0044] Take the scallion leaves, dry them in the shade, and crush them through a 20-mesh sieve. Soak in 60% ethanol at a sample leaf ratio of 1:35, extract at 50°C or 90°C (selected according to different needs) for 3.5 hours, add activated carbon powder, filter, and evaporate the liquid to dryness with a rotary evaporator to obtain the coarse onion. Dried samples of flavonoids can be further developed.
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Abstract
The invention discloses a method for extracting flavonoids from Allium mongolicum Regel leaves. The method comprises steps of: crushing Allium mongolicum Regel leaves dried in the shade by a grinder; sieving; adding the crushed leaves into a hot reflux extractor; soaking the crushed leaves with an ethanol solution; conducting heat reflux extraction by heating with a water bath for 3-4 h for extraction; adding an activated carbon powder into a triangular flask; filtering by a filter paper; and evaporating the liquid to dryness by a rotary evaporator to obtain an Allium mongolicum regel crude flavonoids dry sample. The method provided by the invention employs several conventional extraction methods of flavonoids, and can realize large-scale enterprization production; besides, the method studies antioxidant capacity of crude flavonoids under different temperatures, and provides the theoretical basis for different needs.
Description
technical field [0001] The invention relates to a method for extracting flavonoids, in particular to a method for extracting flavonoids from leaves of shallot. Background technique [0002] Plants are a huge natural treasure house of bioactive compounds, producing more than 400,000 secondary metabolites. Among them, flavonoids are a kind of phenolic compounds widely distributed in the plant kingdom. A large number of studies have shown that flavonoids have the functions of scavenging free radicals, anti-oxidation, anti-mutation, anti-tumor, anti-bacterial, anti-virus, regulating immunity, preventing and curing arteriosclerosis, and lowering blood sugar. The current research on flavonoid extraction is mainly concentrated in Rutaceae, Labiatae, Fabaceae, Umbelliferae, Ginkgoceae and Compositae. Although there have been reports of flavonoids in Allium plants for a long time, there are few specific studies. Only a small amount of flavonoid glycosides have been isolated from s...
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IPC IPC(8): A61K36/8962A61P39/06G01N21/31A61K127/00
Inventor 杨忠仁庞杰张凤兰郝丽珍黄修梅
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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