Enzymolysis method for processing tobacco stems
A tobacco stem and enzymatic hydrolysis technology, which is applied in the field of tobacco, can solve the problems of consuming production line storage space, complicating the process connection, and limiting effective application, etc., and achieve the effects of improving efficiency, improving smoking quality, and improving quality
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Embodiment 1
[0028] Stem washing: install a transmission device in the stem washing tank so that the tobacco stems and the washing water flow relative to each other at 0.15m / s, the mass ratio of the tobacco stems to the washing water is 1:20, and the washing water contains 0.2 % stem-washing compound enzyme (composed of 50% pectinase and 50% xylanase), and the water temperature was kept at 55°C during the stem-washing process. After washing the stems, the tobacco stems are further subjected to primary storage, moistening and secondary storage for a total of 4 hours.
[0029] Stem pressing and cutting: before pressing and cutting, the tobacco stems are heated and the tobacco stems are cooled respectively. The temperature is increased by HT, and the steam flow rate is controlled to make the temperature of the tobacco stem 45-55°C, which is lower than the 70°C of the conventional process.
[0030] (3) The second step of enzymatic hydrolysis reaction:
[0031] Aroma-increasing complex enzyme...
Embodiment 2
[0037] The shredded stems of sample No. 1 were used as a control test, using one-step enzymatic hydrolysis, and the enzymes used were 30% pectinase + 40% hemicellulase + 30% cellulase. The test results are shown in Table 2.
[0038] Table 2. Contents of cellulose, acid-insoluble lignin, and acid-soluble lignin in tobacco stems after one-step enzymatic hydrolysis
[0039] Sample number Comprehensive cellulose (%) Acid insoluble lignin (%) Acid-soluble lignin (%) Soluble protein (%) 1 38.65 5.61 0.41 2.18
Embodiment 3
[0041] After the No. 1 sample of Example 1 was subjected to two-step enzymatic hydrolysis treatment, it was subjected to sensory evaluation, and compared with the untreated control shredded stem. The results are shown in Table 3.
[0042] Table 3 Comparison of sensory evaluation results before and after two-step enzymatic hydrolysis treatment
[0043] sample aroma coordination irritant Miscellaneous aftertaste Control Stem light aroma, rough Shang coordination slightly have Clean and comfortable Enzyme Added Shredded Stem Strong aroma, slightly rough more coordinated slight slightly Cleaner and more comfortable
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