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Cultivating method for obligate parthenogenesis rotifera population

A breeding method and population technology, applied in animal husbandry, etc., can solve the problems of limiting rotifer production, limiting population reproduction efficiency, and occupying breeding space, so as to improve rotifer production efficiency, reduce the volume of breeding water, and increase the cultivation density. Effect

Inactive Publication Date: 2013-04-24
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The emergence of sexual reproduction greatly limits the efficiency of population reproduction, and there is even a danger of collapse, thereby limiting the output of rotifers, which brings about the problem of insufficient supply of live bait
And in order to avoid the sexual reproduction of rotifers, it is necessary to limit the culture density of the rotifers to 20 per milliliter or less, and then it is necessary to increase the water for cultivating rotifers, which takes up a lot of breeding space and increases the cost of raising seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] a. Take 1-2 kg of freshwater fish pond bottom mud, add 1000 ml of hypertonic solution (a mixture of saturated saline and saturated sucrose water), stir well and let it stand for 10-15 minutes, gently dip a plastic plate to pick up the floating water on the surface A number of dormant eggs of Brachionus calyxae were placed in 500 ml of 0.1pp lake water (culture solution) filtered through a 0.45 micron filter membrane, and incubated under the environmental conditions of temperature 24 degrees, light 3000lx, and photoperiod 16:8 for 3 ~5 days;

[0017] b. Expand the hatched rotifers into a single body, feed the culture solution of Nannochloropsis with a density of 1 million cells / ml, the temperature is 24 degrees, the light is 3000lx, and the photoperiod is 16:8;

[0018] c. Pick 100 newly hatched individuals and carry out expanded culture respectively to form 100 clonal populations, each clonal population has a culture fluid volume of 2000 ml, and the culture fluid and b...

Embodiment 2

[0028] a. Take 1-2 kg of seawater river crab nursery pond bottom mud, add 1000 ml of hypertonic solution (a mixture of saturated saline and saturated sucrose water), stir evenly and let it stand for 10-15 minutes, gently dip a plastic plate to pick up the floating water on the surface A number of resting eggs of Brachionus rugosa were placed in 500 ml of 15ppt seawater (culture solution) filtered through a 0.45 micron filter membrane, and incubated under the environmental conditions of temperature 24 degrees, light 3000lx, and photoperiod 16:8 for 3~ 5 days;

[0029] b. Expand the hatched rotifers into a single body, feed the Nannochloropsis culture solution with a density of 2 million cells / ml, the temperature is 24 degrees, the light is 3000lx, and the photoperiod is 16:8;

[0030] c. Pick 100 newly hatched individuals and carry out expanded culture respectively to form 100 clonal populations, each clonal population has a culture fluid volume of 2000 ml, and the culture flu...

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Abstract

The invention discloses a cultivating method for an obligate parthenogenesis rotifera population by using chemical drug induction, microwave irradiation mutation, ultraviolet radiation mutation and manual breeding. In a breeding process of the cultivated rotifera population, even if in a nutrient solution with density of 800-1000 / milliliter, parthenogenesis is conducted, and sexual propagation is not conducted, so that rotifera cultivating density can be greatly increased, rotifera production efficiency is improved, cultivation water volume is reduced, production cost is reduced, the cultivating method can be applied to indoor rotifera high-density cultivation, is also suitable for outdoor open pool cultivation propagation of rotifera, and can provide sufficient live food organism for cultivating of offspring seeds and larva of aquatic product economical cultivation animals.

Description

technical field [0001] The invention relates to a method for cultivating rotifer populations, in particular to a method for cultivating rotifer populations capable of significantly increasing the culture density of rotifers, improving the production efficiency of rotifers, reducing the volume of culture water, and reducing production costs. . Background technique [0002] Regardless of whether it is seawater fish or freshwater fish, during the period of nursery and larvae cultivation, rotifers are mainly used as live bait. Based on the calculation that each fry feeds 20-50 rotifers per day, a small and medium-sized nursery enterprise that produces 50 million fish fry needs to have a daily demand of 1.5-2.5 billion rotifers, according to the ratio of 1:1. Calculated by harvesting rotifers, nursery units need to produce 2.5 to 5 billion rotifers per day. Rotifers include Brachionus rugosa in seawater and Brachionus calyxae in freshwater. They are periodic parthenogenetic ani...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 殷旭旺赵乃锡谭冰冰朱美桦周艳春
Owner DALIAN OCEAN UNIV
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