Method for improving test-crossing and back-crossing efficiency during breeding process of hybrid rice
A hybrid rice, high-efficiency technology, applied in the agricultural field, can solve the problems of no-grave protection, adverse environmental impact, increased workload and cost, etc.
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[0030] The technical solutions of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0031] A method for improving testcross and backcross efficiency in hybrid rice breeding process, comprising the following steps:
[0032] (1) Preparation and processing of test cross male sterile lines
[0033] After 16:00 in the afternoon, the flowering of the hybrid rice maintainer and restorer lines will end. Select the ear of the sterile line that will bloom or will bloom tomorrow and the day after tomorrow and has no pests and diseases. Cut off part of the flag leaves, and cut off the flowers that have bloomed or are too young. ;
[0034] Shake the treated CMS rice ears lightly to disperse the spikelets, and use a watering can filled with 4mmol / L methyl jasmonate and 0.01% Tween treatment solution to completely spray the rice ears, subject to the absence of drops The formula of the treatment solution...
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