Molecular motor biosensor kit for detecting salmonella

Abstract

A molecular motor biosensor kit for detecting salmonella belongs to the field of food microorganism rapid detection, and mainly solves the problem of a long period (5-6 days) for traditional detection methods. The core technology of the invention is that since F0F1-ATP synthase can rotate rapidly, coupling between catalytic sites and proton transport is established through rotation by using the F0F1-ATPase molecular motor biosensor on a chromatophore. Firstly, an invA probe is connected with an epsilon subunit of the ATP synthase; a sample to be detected and a negative control are respectively combined with the biosensor; the ATP yield after the catalysis of ATP synthesis for 30 min is compared; the amount of ATP synthesis can be measured by the H+ amount in the environment, and the amount of H+ can be obtained by fluorescence intensity reflected by H-DHPE. The kit of the invention can rapidly and sensitively detect salmonella in samples to be detected with high throughput.

Description

technical field [0001] The invention relates to a rapid detection technology for food microorganisms. Background technique [0002] Traditional pathogen detection methods require non-selective enrichment, selective enrichment, separation, screening, and identification for each inspection item. It usually takes 5 days to issue a test report, which seriously affects the quality and shelf life of the goods. Some new technologies such as PCR technology, immunological detection technology, biochip technology and other detection methods still rely on traditional detection steps, that is, they need to be enriched, which is time-consuming and labor-intensive. It usually takes several days to complete a detection. This not only increases the workload of the laboratory, but also is not conducive to the monitoring of the production process in the food industry, the quality control of the final product, and the management and control of food safety by government departments. Due to the...

AI Technical Summary

Problems solved by technology

[0002] Traditional pathogen detection methods require non-selective enrichment, selective enrichment, separation, screening, and identification for each inspection item. It usually takes 5 days to issue a test report, which seriously affects the quality and shelf life of the goods.

Some new technologies such as PCR technology, immunological detection technology, biochip technology and other detection methods still rely on traditional detection steps, that is, they need to be enriched, which is time-consuming and labor-intensive. It usually takes several days to complete a detection

This not only increases the workload of the laboratory, but also is not conducive to the monitoring of the production process in the food industry, the quality control of the final product, and the management and control of food safety by government departments.

Images