Agent for disinfection of operating room
An operating room and pharmacy technology, applied in the field of clinical nursing, can solve the problems of bacterial infection, weak physique of surgical patients, etc., and achieve the effects of cheap price, simple prescription and clear curative effect.
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Embodiment 1
[0010] Weigh 50 grams of dried ginger, 80 grams of white mustard seeds, 30 grams of licorice, 20 grams of kansui, 30 grams of mountain bean root, 50 grams of Xiaotongcao, 20 grams of Acanthopanax, 120 grams of Fangfeng, 40 grams of Albizia Julibrissin, 170 grams of Notopterygium , 40 grams of Baibu, 130 grams of cinnamon, 60 grams of gallinaceous gold, 20 grams of tangerine peel, 20 grams of Ophiopogon japonicus and 50 grams of cocklebur, add 1000 ml of water, and place in a sealed flask. Heating in a water bath at 30° C. for 5 hours, cooling to room temperature, filtering, collecting the filtrate, concentrating to 500 ml, and putting it into a sprayer to make a spray.
experiment example 1
[0011] Experimental example 1: antibacterial test
[0012] Adopt classic filter paper method in this field to carry out bacteriostasis test, wherein:
[0013] 1. Materials
[0014] 1.1 Bacteria to be tested
[0015] Staphylococcus aureus (Staphylococcus aureus) ATCC 25923, Candida albicans (Candida albicans) ACTT 12228 were purchased from China Institute for the Control of Biological Products.
[0016] 1.2 Culture medium
[0017] Nutrient agar and nutrient broth were purchased from Beijing Zhongke Chenyu Experimental Equipment Co., Ltd.
[0018] 1.3 Test method
[0019] Inoculate Staphylococcus aureus and Candida albicans on the nutrient solution plane of the agar plate, and spread evenly and densely when inoculating. Take the aseptic circular filter paper sheet with tweezers, spray the spray prepared in Example 1 until completely wetted, and stick it on the nutrient solution plane of the agar plate inoculated with bacteria. Place the agar plate in a 37°C incubator and i...
experiment example 2
[0022] Experimental example 2: Antibacterial test in the operating room
[0023] In the indoor area is equal to 30m 2 Spray 100 ml of the spray prepared in Example 1 evenly in the operating room. 12 hours, 24 hours, and 48 hours after spraying, use the plate sedimentation method to sample, set 3 sampling points, and take 3 points on a diagonal line, that is, 1 point in the middle and 1 point at each end 1m away from the wall , and repeat 10 times. After sampling, culture the culture medium in a 35°C incubator for 48 hours, observe the number of bacterial colonies on the culture medium in a bright place, and calculate the number of bacteria according to the following formula. The amount of bacteria in the air (cfu / m 3 ) = average number of colonies per dish × 50000 / plate exposure time × plate area. All bacteria were identified in accordance with the "National Clinical Testing Procedures".
[0024] 12 hours, 24 hours, 48 hours after spraying, the number of bacteria (cfu / m ...
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