Rapid after-ripening and germination method of pulsatilla koreana seeds

Abstract

The invention relates to a plant propagation technology and provides a rapid after-ripening and germination method of pulsatilla koreana seeds. The rapid after-ripening and germination method comprises the following steps of: immersing the pulsatilla koreana seeds by utilizing a solution mixed by KT with the concentration of 2.10-2.30mg / L, GA3 with the concentration of 2.90-3.20mg / L and 2,4-D with the concentration of 0.30-0.60mg / L; uniformly mixing the seeds with river sand and putting the mixture into a wood groove; placing the wood groove into a room to carry out poikilothermic treatment for 24-28 days, wherein the poikilothermic treatment amplitude and time are as follows: the temperature at daytime is 252 DEG C and the treatment time is 14 hours; and the temperature at night is 102 DEG C and the treatment time is 10 hours; and treating for 24-28 days and directly sowing the seeds. The pulsatilla koreana seeds can germinate after being treated by the method and sowed for 10-14 days; and the germination rate is more than 99.9%. The rapid after-ripening and germination method of the pulsatilla koreana seeds has the characteristics of short seed treatment time, strong maneuverability, very high germination rate and the like. The rapid after-ripening and germination method belongs to a rapid after-ripening and efficient seedling raising technique and can be popularized and applied to industrial seedling raising and scaled cultivation of pulsatilla koreana.

Description

[0001] technical field [0002] The invention relates to a plant propagation technique, that is, a method for quickly post-ripening and germination of Pulsatilla pulsatillae seeds. Background technique [0003] In the prior art, Korean Pulsatilla [ Pulsatilla cernua (Thunb.) Bercht. et Opiz.], Ranunculaceae Pulsatilla is a perennial herbaceous plant, the root contains Clematis epibiose saponin, Clematis disaccharide saponin, saponin Ⅱ and saponin Ⅲ and other important chemical components, with laxative Fire detoxification, pain relief, diuresis and swelling effects, etc. It is mainly used to treat amoebic dysentery, malaria, gold sores, women's amenorrhea, wind-fire toothache, limb joint pain, baldness and edema. disease; it can also be used as insecticide and botanical pesticide. Pulsatilla pulsatillae is very rare in Changbai Mountains. It is a precious and authentic medicinal plant in Changbai Mountains. It is designated as a provincial-level key protected plant in the a...

AI Technical Summary

Problems solved by technology

[0004] Korean pulsatilla is mostly distributed in the alpine regions with a short frost-free period in Northeast China. In autumn, after the seeds are harvested, the seed embryos in the seeds are in the middle stage of the cotyledon embryo, and post-ripening treatment is required, otherwise the germination of the seeds after sowing is extremely low, and asexual reproduction such as split roots The rate is extremely low, and wild resources are threatened with extinction, and large-scale artificial cultivation, development and utilization are greatly restricted

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