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Nano-zinc oxide modified immunocapillary and its preparation method and application

A nano-zinc oxide and capillary technology, applied in the direction of measuring devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of low sensitivity of immune devices, poor signal uniformity, and difficult application of immune analysis, etc., to achieve sensitive immune analysis and improve the surface Density, the effect of convenient optical detection

Active Publication Date: 2016-05-18
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main method of immunoassay is enzyme-linked immunosorbent assay, which is time-consuming, expensive, requires large-scale instruments and skilled operation techniques, and is not suitable for rapid, on-site analysis and large-scale immune screening.
In addition, there are some portable immune analyzers on the market, such as immune test strips, which can provide rapid on-site immune analysis, but this type of immune analyzer can only perform qualitative or semi-quantitative detection, and cannot provide quantitative analysis.
[0003] In 2006, Omowunmi A. Sadik and Jason Karasinski of the United States used glass capillary tubes to prepare immunodetection devices (United States Patent: 7708944). This immunodetector can be used for quantitative analysis, but the immune device has low sensitivity and poor signal uniformity, which is difficult to apply to actual immunoassays. analyze

Method used

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  • Nano-zinc oxide modified immunocapillary and its preparation method and application

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Embodiment 1

[0034] The preparation method of the immune capillary modified by nano-zinc oxide, the specific steps are as follows:

[0035] (1) Take a newly prepared potassium permanganate solution with a concentration of 5mM and inject it into the glass capillary, soak it for 20 minutes to activate the glass capillary; then rinse it with deionized water, and then put it into the glass capillary containing 5% (v / v) ethanolamine and 5% (v / v) In the 50mM zinc nitrate solution of ammoniacal liquor, grow in 80 ℃ of water baths for 50 minutes, form zinc oxide nanorod on capillary surface, the tube wall of capillary is photographed with scanning electron microscope, its result is as follows figure 1 shown.

[0036] (2) Rinse the glass capillary treated in step (1) with deionized water, then inject 3% (v / v) ethanol solution of 3-epoxypropyloxypropyltrimethoxysilane after drying, soak for 2 Hours, 3-epoxypropyloxypropyltrimethoxysilane and ZnO nanostructures and capillary inner walls formed sili...

Embodiment 2

[0040] The preparation method of the immune capillary modified by nano-zinc oxide, the specific steps are as follows:

[0041] (1) Take a newly prepared potassium permanganate solution with a concentration of 1mM and inject it into the glass capillary, soak for 30 minutes to activate the glass capillary; then rinse with deionized water, put 1% (v / v) ethanolamine and 1% (v / v ) in a 10 mM zinc nitrate solution of ammonia water and grown in a water bath at 65° C. for 60 minutes to form zinc oxide nanorods on the surface of the capillary.

[0042](2) Rinse the glass capillary treated in step (1) with deionized water, then inject 1% (v / v) ethanol solution of 3-epoxypropyloxypropyltrimethoxysilane after drying, soak for 1 After 3-epoxypropyloxypropyltrimethoxysilane and the ZnO nanostructure and the inner wall of the capillary formed a silicon-oxygen covalent bond, rinsed with ethanol and annealed in vacuum at 110°C for 3 hours, then added 50 μg / mL mouse anti-human CEA (carcino-em...

Embodiment 3

[0046] The preparation method of the immune capillary modified by nano-zinc oxide, the specific steps are as follows:

[0047] (1) Prepare the colloidal solution of zinc oxide nanoparticles according to the prior art (for the preparation method see: ContinuousSizeTuningofMonodisperseZnOColloidalNanocrystalClustersbyaMicrowave-PolyolProcessandTheirApplicationforHumiditySensing, XianluoHu, JingmingGong, LizhiZhangandJimmyC.Yu.Adv.Mater.2008, 20, (24), 484 The solution is injected into the glass capillary, soaked for 20 minutes, and then the colloidal solution is discharged. The steps of soaking and discharging can be repeated several times to increase the adsorption capacity of zinc oxide nanoparticles, and then calcined at 150°C for 60 minutes. taken by electron microscope, the results are as follows Figure 5 shown.

[0048] (2) Inject the glass capillary treated in step (1) into 1% (v / v) ethanol solution of 3-epoxypropyloxypropyltrimethoxysilane, soak for 3 hours, and 3-epox...

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Abstract

The invention relates to nano zinc oxide modified immune capillary and its preparation method and application. The immune capillary is formed by growing nano zinc oxide on the inner surface of glass capillary, and then combining detection probes on the nano zinc oxide. The huge specific surface area can effectively increase the surface density of antibody proteins. At the same time, nano-zinc oxide has unique physical properties to amplify the fluorescent signal of fluorescently-labeled proteins; while the capillary can provide the microchannels required for flow detection and has optical transparency, so as to realize Rapid, quantitative immunoassays can be used for early screening of malignant tumors and food safety analysis.

Description

technical field [0001] The invention relates to an immunodetection device, in particular to an immunocapillary modified by nanometer zinc oxide, and to a preparation method and application of the capillary immunocapillary. Background technique [0002] Immunoassay is a quantitative and qualitative detection method for various substances such as drugs, hormones, proteins, microorganisms, etc. through the specific binding between antigens and antibodies. It is widely used in disease diagnosis, environmental monitoring, food safety and other fields. For example, immunoassay is used to detect specific tumor markers in serum, which is an effective and non-invasive method for early diagnosis and screening of tumors. At present, the immunoassay method mainly adopts enzyme-linked immunosorbent assay, which is time-consuming, expensive, requires large-scale instruments and skilled operation techniques, and is not suitable for rapid, on-site analysis and large-scale immune screening. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/531G01N21/64
Inventor 李长明胡卫华刘英帅
Owner SOUTHWEST UNIV
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