Brevibacillus borstelensis strain having capability of degrading thioanisole and application thereof
A technology of Brevibacillus brevis and thioanisole is applied in the field of microorganisms, which can solve the problem that Brevibacillus potsdam is not found to degrade thioanisole and the like, and achieve the effect of efficiently degrading thioanisole
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Embodiment 1
[0021] The Brevibacillus Potsdamer strain capable of degrading sulfide anisole in the present invention is obtained by separating and purifying the bottom mud of a certain river in Guangzhou City, Guangdong Province. Its separation and purification method is as follows: the acclimatization medium used is inorganic salt medium (contain the following components in every liter of inorganic salt medium: K 2 HPO 4 ·3H 2 O 1.2g, KH 2 PO 4 1.2g, NH 4 Cl 0.4g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.01g, CaCl 2 2H 2 O 0.2g, MnSO 4 4H 2 O 0.2g, CuSO 4 2H 2 O 0.01g, ZnSO 4 ·7H 2 O 0.2g, CoCl 2 ·6H 2 O 0.09g, Na 2 MoO 4 2H 2 O 0.12g, H 3 BO 3 0.006g). First, add 3 g of sludge to the inorganic salt medium containing 10 mg / L sulfide anisole, and after acclimatization for 7 days at 37°C, transfer it into the inorganic salt medium containing 20 mg / L sulfide anisole at an inoculum size of 5% by volume, and then acclimate For one week, gradually increase the concentration...
Embodiment 2
[0055] The Brevibacillus Potsdam GIGAN1 screened by the present invention has better degradation ability to sulfide anisole:
[0056] Weigh K 2 HPO 4 ·3H 2 O 1.2g, KH 2 PO 4 1.2g, NH 4 Cl 0.4g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.01g, CaCl 2 2H 2 O 0.2g, MnSO 4 4H 2 O 0.2g, CuSO 4 2H 2 O 0.01g, ZnSO 4 ·7H 2 O 0.2g, CoCl 2 ·6H 2 O 0.09g, Na 2 MoO 4 2H2O 0.12g, H 3 BO 3 0.0.06g, then dilute to 1L with deionized water, pack 100ml / bottle into 250ml shaker flasks, sterilize at 121°C for 30min, take it out, add thioanisole and thioanisole under sterile conditions The concentration of ether is 2mg / L, 4mg / L, 8mg / L, 16mg / L, 24mg / L in turn. Inoculate the GIGAN1 strain preserved on the slant (the slant medium is beef extract peptone medium) into the beef extract peptone medium for culture, and activate the bacteria in a shaker at 30°C and 220r / min for 15h, with a volume percentage of 5% The amount of inoculum was inserted into the inorganic salt medium containi...
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