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Preparation method of 1,2,3,4,6-pentagalloylglucose reference substances

A technology for pentagalloyl-glucose and reference substance, which is applied in the field of preparation of extraction and separation of 1,2,3,4,6-pentagalloyl-glucose reference substance, can solve the problems of difficulty in separation and preparation, and achieves low separation cost and exclusive Strong, easy-to-follow effects

Inactive Publication Date: 2015-01-07
BEIJING UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, it is of great significance to be able to provide a method for preparing a large amount of 1,2,3,4,6-pentagalloyl glucose. In the study, it was found that although 1,2,3,4,6-pentagalloyl glucose is present in the traditional Chinese medicine gall The content of 1,2,3,4,6-pentagalloylglucose from the traditional Chinese medicine Radix Paeoniae Alba is relatively simple due to the existence of other similar chemical components in gallnut, which brings certain difficulties to the separation and preparation. The invention provides a method for preparing 1,2,3,4,6-pentagalloyl glucose from the traditional Chinese medicine Radix Paeoniae Alba

Method used

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  • Preparation method of 1,2,3,4,6-pentagalloylglucose reference substances

Examples

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Effect test

Embodiment 1

[0029] (1) Grinding Radix Paeoniae Alba into coarse powder, according to the weight ratio of Radix Paeoniae Alba: 80% ethanol aqueous solution = 1:12, refluxing and extracting for 4 hours, recovering the solvent from the filtrate, and obtaining a liquid extract with a specific gravity of 1.15 at room temperature;

[0030] (2) Dilute the above liquid extract of Radix Paeoniae Alba with water at a weight ratio of 1:8, enrich the diluted solution through a macroporous adsorption resin column, elute with 50% ethanol aqueous solution as a desorbent, collect the eluent, and recover Solvent, obtain normal temperature specific gravity 1.12 concentrate;

[0031] (3) Dilute the above concentrated solution of Radix Paeoniae Alba with water at a weight ratio of 1:6, enrich the diluted solution through a macroporous adsorption resin column, elute with 15% ethanol aqueous solution as a desorbent, collect the eluent, and polymerize Amide thin-layer chromatography detection, solvent recovery,...

Embodiment 2

[0035] (1) Grinding Radix Paeoniae Alba into coarse powder, according to the weight ratio of Radix Paeoniae Alba: 60% ethanol aqueous solution = 1:15, refluxing and extracting for 2 hours, recovering the solvent from the filtrate, and obtaining a liquid extract with a specific gravity of 1.16 at room temperature;

[0036] (2) Dilute the above liquid extract of Radix Paeoniae Alba with water at a weight ratio of 1:10, enrich the diluted solution through a macroporous adsorption resin column, elute with 50% ethanol aqueous solution as a desorbent, collect the eluent, and recover Solvent to obtain a concentrated solution with a specific gravity of 1.14 at normal temperature;

[0037] (3) Dilute the above concentrated solution of Radix Paeoniae Alba with water at a weight ratio of 1:6, enrich the diluted solution through a macroporous adsorption resin column, elute with 30% ethanol aqueous solution as a desorbent, collect the eluent, and polymerize Amide thin-layer chromatography ...

Embodiment 3

[0041] (1) Grinding Radix Paeoniae Alba into coarse powder, according to the weight ratio of Radix Paeoniae Alba: 40% ethanol = 1:20, refluxing and extracting for 3 hours, recovering the solvent from the filtrate, and obtaining a liquid extract with a specific gravity of 1.15 at room temperature;

[0042] (2) Dilute the above liquid extract of Radix Paeoniae Alba with water at a weight ratio of 1:6, enrich the diluted solution through a macroporous adsorption resin column, elute with 60% ethanol as the desorbent, collect the eluent, and recover the solvent , to obtain a concentrated solution with a specific gravity of 1.15 at room temperature;

[0043] (3) Dilute the above-mentioned white peony concentrate with water at a weight ratio of 1:8, enrich the diluted solution through a macroporous adsorption resin column, and elute with 30% ethanol as a desorbent, collect the eluate, polyamide Thin-layer chromatography detection, solvent recovery, to obtain 1,2,3,4,6-pentagalloyl gl...

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Abstract

The invention discloses a preparation method of 1,2,3,4,6-pentagalloylglucose reference substances, belonging to the technical field of extraction and purification of traditional Chinese medicine and natural product reference substances. The method comprises the following steps of: crushing white peony root herbs, carrying out reflux extraction and concentration on the crushed white peony root herbs, adding water into the obtained object to dilute, carrying out macroporous adsorptive resin enrichment and desorption on the diluted object two times, and carrying out gel column chromatographic separation on the obtained product so as to obtain a 1,2,3,4,6-pentagalloylglucose reference substance; and through carrying out detection by using a high-performance liquid chromatography, the content can reach more than 98%. The method is lower in cost, simple in steps and strong in specificity.

Description

technical field [0001] The invention relates to a preparation method for extracting and separating 1,2,3,4,6-pentagalloyl glucose reference substance from Radix Paeoniae Alba, which belongs to the technical field of extraction and purification of reference substances of traditional Chinese medicine and natural products. technical background [0002] Paeoniae Alba is the dried root of Paeonia lactiflora Pall., a plant of Ranunculaceae. Bitter in the mouth, sour, slightly cold, returns liver, spleen meridian, has calming liver and relieving pain, nourishing blood and regulating menstruation, astringing yin and antiperspirant effect. For headaches, dizziness, spasms and pains in limbs, irregular menstruation, spontaneous sweating, night sweats and other diseases. Radix Paeoniae Alba is commonly used in traditional Chinese medicine, and its chemical composition mainly contains terpenoids and phenolic compounds such as paeoniflorin and hydroxypaeoniflorin. [0003] 1,2,3,4,6-Pe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H13/08C07H1/08
Inventor 李霄张金玲李泽琳艾萍王小虎
Owner BEIJING UNIV OF TECH
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