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Beggarweed extractive and general flavone and rhizoma kaempferiae glycosides separated from the beggarweed extractive and medical application thereof

A technology for extracts and total flavonoids is applied in the field of medicine and achieves the effects of obvious effect and simple preparation method.

Inactive Publication Date: 2013-04-03
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the prevention and treatment of osteoporosis by the extracts of M. chinensis, total flavonoids or monomeric compounds of kaempferin

Method used

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  • Beggarweed extractive and general flavone and rhizoma kaempferiae glycosides separated from the beggarweed extractive and medical application thereof
  • Beggarweed extractive and general flavone and rhizoma kaempferiae glycosides separated from the beggarweed extractive and medical application thereof
  • Beggarweed extractive and general flavone and rhizoma kaempferiae glycosides separated from the beggarweed extractive and medical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 prepares the extract and kaempferenin monomer compound of Rhizoma chinensis

[0021] 20kg of the whole herb of Yuanlingyeshan leech, crushed. It is decocted in conventional water, the ratio of solid to liquid is 1:15 (v / v), the extraction time is 2 hours, and the number of extractions is 3 times. The extract was filtered hot, and the filtrates were combined and evaporated to dryness under reduced pressure to obtain 3 kg of extract extract, with an extract yield of 15%. Suspend the extract extract with 1 times the amount of water (3L), and successively add petroleum ether (3L×3), dichloromethane (3L×3), ethyl acetate (3L×3) and n-butanol (3L×3 ) equal-volume extraction, take the n-butanol extract and concentrate it under reduced pressure to form a liquid extract, add 1.5 times column chromatography silica gel and mix well, load the sample by dry method, wash with dichloromethane:methanol at a ratio of 10:1 to 5:1 Eluate and elute, collect the eluent, evapo...

Embodiment 2

[0022] Embodiment 2 prepares the total flavonoids of Cyclops chinensis

[0023] 15kg of dried whole grass of Cyclops chinensis, crushed, added 8 times the volume of 95% ethanol, reflux extraction, the extraction time was 3 hours, and the number of extractions was 3 times. The extract is filtered hot, and the filtrates are combined and then concentrated under reduced pressure to a liquid extract, which is the extract of M. chinensis. Add the extract of Lemonia chinensis to the pretreated AB-8 macroporous adsorption resin, wherein the weight ratio of macroporous resin to crude drug of the original medicinal material is 1:1. First eluted with 5 times of column water until colorless, then eluted with 20% ethanol, 30% ethanol, 60% ethanol, 80% ethanol in turn, collected 30-60% of the eluate, and washed the After dehydrating and evaporating to dryness, 18.1 g of total flavonoids of M. chinensis were obtained. Using the kaempferol prepared in Example 1 as a control, the total flavo...

Embodiment 3

[0024] Example 3: Preparation of the extract of the leech and the total flavonoids of the leech

[0025] 15kg of dried whole grass of Cyclops chinensis, crushed, added 8 times the volume of 80% ethanol, reflux extraction, the extraction time was 3 hours, and the number of extractions was 3 times. After hot filtration, the filtrates are combined and then concentrated under reduced pressure to a liquid extract, which is the extract of M. chinensis. Add the extract of Leedia chinensis to the pretreated polyamide resin of 60-100 mesh, wherein the weight ratio of the resin to the crude drug of the original medicinal material is 1:1. First eluted with water until it was colorless, then eluted with 10% ethanol, 30% ethanol, 60% ethanol, and 80% ethanol in sequence, and collected the eluents eluted with 30% ethanol, 60% ethanol, and 80% ethanol respectively. Taking kaempferol as a control, the total flavonoid content was measured by ultraviolet spectrophotometry (341nm), and the resu...

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Abstract

The invention relates to the field of medical technology, in particular to the application of desmodium podocarpum DC extractive and general flavone and rhizoma kaempferiae glycosides monomeric compound separated from the desmodium podocarpum DC extractive in preparation of medicine or food for preventing and curing osteoporosis. The desmodium podocarpum DC dried herb is taken as the raw material for extraction and separation so as to obtain the desmodium podocarpum DC extractive, and the desmodium podocarpum DC extractive is used for preparing general flavone of the desmodium podocarpum DC and the rhizoma kaempferiae glycosides monomeric compound is separated from the desmodium podocarpum DC. In vitro and vivo experiments show that the desmodium podocarpum DC extractive and the general flavone or the rhizoma kaempferiae glycosides monomeric compound of the desmodium podocarpum DC have good biological activity in anti-osteoporosis, thus being used for preparing medicine or food for preventing and curing osteoporosis. The preparation method is simple, and provides new medicine source for anti-osteoporosis.

Description

technical field [0001] The invention relates to the technical field of medicines, and relates to the application of the extract of the leeches and the total flavonoids and kaempferrin monomer compounds of the leeches isolated therefrom in the preparation of medicines or foods for the prevention and treatment of osteoporosis. Background technique [0002] Osteoporosis (Osteoporosis, OP) is a common disease and frequently-occurring disease. At present, there are about 200 million people in the world suffering from osteoporosis, and its incidence rate has jumped to the seventh among various common diseases in the world. At present, chemical drugs for treating osteoporosis mainly use bone formation stimulators such as estrogen bone resorption inhibitors or androgen, and long-term use has relatively large side effects. Finding effective extracts, effective parts and monomer compounds from natural plants to treat osteoporosis with less side effects has become a research hotspot. ...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61K31/7048A61P19/10A23L1/29C07H17/07C07H1/08A23L33/00
Inventor 郑承剑秦路平马学琴张巧艳韩婷张宏
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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