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Marine bacterium preparation for preventing banana vascular wilt

A technology of banana fusarium wilt and marine bacteria, which is applied in the directions of bacteria, fungicides, biocides, etc., can solve the problems of difficult control effect of chemical agents, affecting people's health, and poor disease prevention effect, achieving human and animal safety and beneficial to agriculture. The effect of pollution-free production and organic production, which is beneficial to the increase of banana production and farmers' income

Inactive Publication Date: 2013-04-03
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the disease is a soil-borne disease, chemical agents are difficult to prevent and control, and long-term use of chemical agents can easily cause pathogenic bacteria to develop resistance, pollute the environment, affect people's health and other adverse effects; now high-yield, high-quality bananas resistant to Fusarium wilt Varieties have not yet come out; in addition, agricultural control measures such as cultivation management, crop rotation, etc. are not effective in disease prevention
It can be seen that there is currently no effective method for the prevention and treatment of the disease

Method used

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  • Marine bacterium preparation for preventing banana vascular wilt
  • Marine bacterium preparation for preventing banana vascular wilt
  • Marine bacterium preparation for preventing banana vascular wilt

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Preparation of Marine Bacteria BaL3 Strain Preparation

[0020] The present invention relates to a kind of marine bacterium that prevents and cures banana wilt and its preparation. Acanthus ilicifolius ) Bacillus amyloliquefaciens isolated from leaves ( Bacillus amyloliquefaciens ). The colonies of this strain on NA medium are light yellow, opaque, rough and dry on the surface, with irregular edges, straight rods, positive Gram staining, spore-forming, spores are oval, mesozoic or nearly mesozoic. The growth temperature range of the strain is 15-50°C, the growth pH range is 5.0-9.0, and it can tolerate 10.0% sodium chloride. The strain can colonize bananas and root soil, and can secrete and produce antibacterial proteins that inhibit a variety of plant pathogenic fungi;

[0021] Preparation of liquid bacterial preparation: Inoculate BaL3 strain on PBY medium (5.0 g beef extract, 10.0 g peptone, 5.0 g NaCl, 5.0 g yeast extract, 10.0 g glucose, pH 7.5, water 1000 m...

Embodiment 2

[0024] Colonization of Marine Bacteria BaL3 Strain in Banana and Rhizosphere Soil

[0025] Take the culture solution of BaL3 strain, and use the watering method to pour the bacterial solution on the roots of banana seedlings. The isolation results show that the strain can be isolated from the banana pseudostem, root surface and rhizosphere soil after 30 days of treatment, indicating that the strain can be It colonizes and survives in the banana pseudostem, root surface and rhizosphere soil for more than 30 days.

Embodiment 3

[0027] Control of indoor potted banana fusarium wilt and growth-promoting effects of marine bacteria BaL3 strain preparation

[0028] Select 6-7 banana tissue culture seedlings with strong leaves, transplant them into round pots (160×220 mm) filled with sterilized soil, and treat them according to the following 6 groups: (A) Immediately after transplanting, use BaL3 Strain liquid preparation (concentration of 10 8 cfu / mL) root irrigation (50 mL / strain), repeat treatment once every 20 days; (B) spray leaves with BaL3 strain liquid preparation before transplanting, and soak roots with BaL3 strain liquid preparation for 2 h during transplanting; (C) ( A) + (B) treatment; (D) Stir the solid preparation of BaL3 strain with soil before transplanting (100 g / plant), and then follow treatment (A); CK1: clean water control; CK2: pathogenic bacteria treatment control. For each treatment of 10 pots (strains), the roots were inoculated with the spore suspension of Fusarium wilt (concen...

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Abstract

The invention relates to marine bacterium preparations for preventing banana vascular wilt and belongs to the field of plant protection. A bacterial strain of marine bacteria BaL3 is Bacillus amyloliquefaciens. The bacterial strain is inoculated to a PBY culture medium to be subjected to shaking culture for 36 to 48 hours at a speed of 180 to 200 r / min at a temperature of 25 to 28 DEG C, so as to obtain a liquid bacterium preparation; and total concentration of viable bacteria in the liquid bacterium preparation is 1*109 to 1*1,010 cfu / ml. The liquid bacterium preparation of the bacterial strain is inoculated to a solid culture medium to be subjected to fermental cultivation for 24 to 48 hours; after the obtained product is dried, a solid bacterium preparation is prepared; and total concetration of viable bacteria in the solid bacterium preparation is 1*109 to 1*1,012 cfu / g. The preparations have a greenhouse control effect of 80 to 100 percent on banana vascular wilt, have a field efficacy of 46.4 percent to 72.3 percent on banana vascular wilt and can promote thickening of cauloid of bananas, green increasing of leaves and early bud drawing. The preparations can be irrigated to roots or sprayed to prevent diseases and can also be used as bio-fertilizers.

Description

technical field [0001] The invention relates to a marine bacterium preparation, in particular to a marine bacterium BaL3 for preventing and treating banana wilt and its preparation, which belongs to the technical field of crop disease prevention and treatment, and is specially used for pollution-free prevention and treatment of banana fusarium wilt. Background technique [0002] Banana wilt, also known as banana Panama disease and banana yellow leaf disease, is caused by Fusarium oxysporum Cuban specialization ( Fusarium oxysporum f .s p. cubes ) caused by destroying banana vascular bundles and causing plant death. The disease is widely distributed in banana planting areas in the world. It also occurs in banana planting areas such as Guangdong, Guangxi, Hainan, Fujian, and Taiwan in my country. It is a serious impact on my country. One of the main factors for the healthy development of the banana industry, so the prevention and control of banana wilt is imminent. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00A01G7/06C12N1/20C12R1/07
Inventor 何红
Owner GUANGDONG OCEAN UNIVERSITY
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