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Method for inducing regeneration of Chinese tallow tree plant by virtue of young embryoid genesis approach

A technology of embryoid body and black tallow, which is applied in the biological field to achieve the effects of avoiding many chimeras, high reproduction coefficient and complete embryoid body structure.

Active Publication Date: 2013-04-03
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Problems solved by technology

However, there is no report on the method of inducing the regeneration of Chinese tallow tree plants through the embryoid body of young embryos.

Method used

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  • Method for inducing regeneration of Chinese tallow tree plant by virtue of young embryoid genesis approach
  • Method for inducing regeneration of Chinese tallow tree plant by virtue of young embryoid genesis approach
  • Method for inducing regeneration of Chinese tallow tree plant by virtue of young embryoid genesis approach

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Embodiment

[0021] A method for inducing the regeneration of Chinese tallow tree plants through the pathway of immature embryoid embryoid body generation. The specific operations are as follows:

[0022] 1. Collect the immature seeds of Chinese tallow tree in mid-July, that is, about one and a half months after pollination.

[0023] 2. After peeling off the green husk, the seeds are surface-sterilized with 75% ethanol for 2-3 minutes, rinsed with sterile water twice, then disinfected with 0.1% mercury chloride for 5-7 minutes, and finally rinsed with sterile water for 5 times to disinfect Shake constantly.

[0024] 3. Take out the immature embryos with endosperm, place them on the embryoid body induction medium for induction after partial damage, and obtain two different forms of embryoid bodies after about 2 months of dark culture ( figure 2 , 3), the induction rate can reach about 90%. Embryoid body induction medium is MS+ 0.1-2.0mg / L 2,4-D or 0.1-2.0mg / L IAA or 0.2-2.0mg / L NAA + 30g...

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Abstract

The invention discloses a method for inducing regeneration of a Chinese tallow tree plant by virtue of a young embryoid genesis approach. The method disclosed by the invention is high in reproduction speed and high in reproduction coefficient (each embryo can produce dozens of and even more seedlings); and fruit characteristics of a plant can be really reflected and inheritable characters are stable, and important technical support is provided for rapid propagation of an excellent mature Chinese tallow tree plant and genetic improvement; and the problem that time and effort are wasted in the traditional Chinese tallow tree plant breeding process is alleviated in certain degree, and the problems of multiple chimeras and unstable inheritable characters as propagation and genetic improvement are carried out on the Chinese tallow tree by virtue of organ generation and indirect callus regeneration approaches are also avoided.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a plant tissue regeneration method, in particular to a method for inducing the regeneration of Chinese tallow tree through the embryoid body generation pathway of immature embryos. Background technique [0002] Sapium sebiferum (Sapium sebiferum) is a deciduous tree of the Euphorbiaceae Sapium genus. It is a widely distributed landscape tree species with high ornamental value, integrating appearance, leaf viewing and fruit viewing; its seeds contain both liquid oil and solid Fat, with an oil content of 42%-53%, is an important industrial woody oil plant; the seeds are black and round, covered with white waxy arils, which can be used to make paints, and the arils are the raw materials for making candles and soaps. The white wax layer on the surface of the seed shell can extract solid oil called tallow fat or shell oil; the black seed shell contains the seed kernel, which can extract liqu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 吴丽芳侯金艳李明浩
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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