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Fusion protein of IBD (Infectious Bursal Disease) VP2 and IL (Interleukin)-2 and application thereof

A fusion protein and virus technology, applied in the direction of antiviral agents, virus antigen components, medical preparations containing active ingredients, etc., can solve the problems of not providing enough protection, and the protection rate is only 10%~70%

Active Publication Date: 2013-03-27
QINGDAO VLAND BIOTECH INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past ten years, due to the emergence of super-virulent strains and variants of chicken infectious bursal disease virus IBDV, antigenic variation and diversity of serotypes, the current commercial vaccines cannot provide sufficient protection. Only 10%~70%

Method used

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  • Fusion protein of IBD (Infectious Bursal Disease) VP2 and IL (Interleukin)-2 and application thereof
  • Fusion protein of IBD (Infectious Bursal Disease) VP2 and IL (Interleukin)-2 and application thereof
  • Fusion protein of IBD (Infectious Bursal Disease) VP2 and IL (Interleukin)-2 and application thereof

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Embodiment Construction

[0011] The main structural protein VP2 protein of IBDV is the most important host protective antigen, while chicken IL-2 has obvious immune enhancement effect. The present invention connects the two genes through a linker by overlapping extension PCR method and clones them into the pET-28a vector Above, BL21(DE3) was transformed, and the VP2-IL2 fusion protein was expressed after being induced by IPTG. The expressed bacteria were homogenized under high pressure and then centrifuged to obtain the supernatant, which was the VP2-IL2 protein solution, which was made into oil by adding mineral oil adjuvant The emulsion vaccine can be used to prevent chicken infectious bursal disease, and its effect is better than that of VP2 subunit vaccine.

[0012] The fusion protein of the present invention will be described in detail below in conjunction with specific examples.

[0013] 1. Screening of VP2 gene

[0014] A pair of primers for amplifying chicken infectious bursal disease virus V...

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Abstract

The invention aims to provide a fusion protein of VP2 protein of an IBD (Infectious Bursal Disease) virus and an IL (Interleukin)-2, wherein the amino acid sequence of the fusion protein is SEQ ID NO: 1. According to the invention, the supervirulent VP2 protein of the IBDV (Infectious Bursal Disease Virus) is expressed with the IL-2 protein with immunological enhancement in a fusion manner, and the vaccine prepared through subcutaneous injection has a strong resistivity to resist the attach of the IBDV for chickens.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering subunit vaccines, and in particular relates to a fusion protein of IBD virus VP2 protein and chicken IL-2 and its application, that is, a chicken infectious bursal disease virus VP2 protein and chicken IL-2 fusion protein and its application . Background technique [0002] Chicken infectious bursal disease (Infectious Bursal Disease, IBD) mainly invades the central immune organ of chickens, the bursa of Fabricius, which leads to immunosuppression, increases the susceptibility of the body to other pathogens, and reduces the reactivity to other vaccines. Foreign scholars The disease is vividly called "AIDS" of chickens. Although scholars from various countries have made great achievements in the research of IBD vaccines, none of them can fundamentally reverse the current situation of the increasingly serious IBD epidemic. In the past ten years, due to the emergence of super-virulent st...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62A61K39/12A61K39/39A61P31/14
Inventor 王雷凌红丽王宏华王睿智
Owner QINGDAO VLAND BIOTECH INC
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