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Preparation method of CT imaging molecular probe of folate receptor targeted tumor

A technology of folic acid and gold ions, used in the preparation of X-ray contrast agents, can solve the problems of uneven particle size and no verification, and achieve the effects of strong absorption, system uniformity and high attenuation ability

Inactive Publication Date: 2013-03-27
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The particle size prepared by the above two methods is not uniform, and it has not been verified whether it can be applied to CT imaging

Method used

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  • Preparation method of CT imaging molecular probe of folate receptor targeted tumor
  • Preparation method of CT imaging molecular probe of folate receptor targeted tumor
  • Preparation method of CT imaging molecular probe of folate receptor targeted tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A preparation method of folic acid coated nano gold, comprising the following steps:

[0039] Weigh 0.0018g of FA, dissolve it in 50mL ultrapure water, add 1mL of 1% HAuCl4 solution dropwise, at this time, FA and HAuCl 4 The molar ratio is 1:6, placed on a heating stirrer, and stirred for more than 5 minutes (the specific time is not required, just mix well). Use NaOH solution with a concentration of 1M and 0.1M to adjust the pH value of the system to about 7.4. At this time, the solution Exactly bright yellow, FA is all dissolved.

[0040] Weigh 0.1g NaBH 4 , dissolved in 2mL ultrapure water, and placed in ice-water mixture. FA and HAuCl 4 Quickly add NaBH dropwise to the mixture 4solution, the solution all presents a deep wine red color, and is stirred rapidly for 2 hours at a stirring speed of about 1200 rpm. After natural cooling, the obtained sample was dialyzed in ultrapure water (substances with a cut-off molecular weight greater than 500D) for 12 hours to r...

Embodiment 2

[0042] A preparation method of folic acid coated nano gold, comprising the following steps:

[0043] Weigh 0.0026g of FA, dissolve it in 50mL of ultrapure water, add 1mL of 1% HAuCl4 solution dropwise, then FA and HAuCl 4 The molar ratio of the solution is 1:4, placed on a heating stirrer, and stirred for more than 5 minutes (the specific time is not required, just mix well). Use the NaOH solution with a concentration of 1M and 0.1M to adjust the pH value of the system to about 7.2. At this time, the solution Exactly bright yellow, FA is all dissolved.

[0044] Weigh 0.12g NaBH 4 , dissolved in 2mL ultrapure water, and placed in ice-water mixture. FA and HAuCl 4 Quickly add NaBH dropwise to the mixture 4 solution, the solution all presents a deep wine red color, and is stirred rapidly for 2 hours at a stirring speed of about 1500 rpm. After natural cooling, the obtained sample was dialyzed for 12 hours to remove unreacted molecules to obtain folic acid-coated gold nanopro...

Embodiment 3

[0046] A preparation method of folic acid coated nano gold, comprising the following steps:

[0047] Weigh 0.0053g of FA, dissolve it in 50mL ultrapure water, add 1mL of 1% HAuCl4 solution dropwise, then FA and HAuCl 4 The molar ratio is 1:2, placed on a heating stirrer, and stirred for more than 5 minutes (the specific time is not required, just mix well). Use NaOH solutions with a concentration of 1M and 0.1M to adjust the pH of the system to about 7.5. At this time, the solution Exactly bright yellow, FA is all dissolved.

[0048] Weigh 0.13g NaBH 4 , dissolved in 2mL ultrapure water, and placed in ice-water mixture. FA and HAuCl 4 Quickly add NaBH dropwise to the mixture 4 solution, the solution all presents a deep wine red color, and is stirred rapidly for 3 hours, and the stirring speed is about 1000 rpm. After natural cooling, the obtained sample was dialyzed for 12 hours to remove unreacted molecules to obtain folic acid-coated gold nanoprobes, and the prepared sa...

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Abstract

The invention discloses a preparation method of a CT imaging molecular probe of folate receptor targeted tumors, which comprises the following steps: dissolving folic acid which is used as a wrapping agent in a solution which contains gold ions and is used for preparing nanogold, adjusting the pH to 7.0-7.5 to obtain a mixed solution, wherein the molar ratio of folic acid to gold ions is 1:2-6; adding a reducing agent into the mixed solution, rapidly stirring and reacting for at least 1 hour, naturally cooling after the reaction, performing dialysis to remove unreacted parts so as to obtain the nanogold coated by folic acid. The nanogold coated by folic acid FA-GNP prepared in the invention has high attenuation capability for X ray, and can be used as a CT imaging contrast agent; the FA-GNP can provide CT images with high contrast for tumors.

Description

technical field [0001] The invention belongs to the technical field of CT molecular imaging, and relates to a preparation method of a folic acid receptor targeting tumor CT imaging molecular probe. Background technique [0002] Malignant tumor is a common disease that seriously endangers human health, and has become one of the main causes of human death. In recent years, with the development of economy and technology, people's living environment and living habits are changing day by day. Tumor has become a problem that endangers human health worldwide and its incidence is on the rise. Compared with developed countries, the mortality rate of malignant tumors in my country is higher than the world average. The time of diagnosis determines the chance of a patient being cured. According to a large number of clinical data statistics, the five-year survival rate of malignant tumors detected in the early stage can reach more than 90%, while that in the late stage is only about 20%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/04
Inventor 姚翠萍张镇西杨洋郭佑民张少娟董艳花王晶梅建生
Owner XI AN JIAOTONG UNIV
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