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Preparation method and application of yolk antibody microcapsule preparation

A yolk antibody and preparation technology, applied in the direction of antibodies, antiviral agents, microcapsules, etc., can solve the problems of complex process, high production cost, degradation, etc., and achieve the effects of good treatment effect, low treatment cost and convenient collection.

Inactive Publication Date: 2013-03-27
TIANJIN JIMINGSHENG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, injection is still the only way of administration of this kind of drug in clinical application, and the outstanding problems are: The half-life of the drug in the blood is short, and it needs long-term frequent injection, which causes a great burden on the body, spirit and economy of the patient. Poor treatment compliance of patients; The production conditions of injections are harsh, and indicators such as heat sources and endotoxins need to be strictly controlled, resulting in complex processes and high production costs; Due to the absence of carrier protection, harsh drug storage and transportation conditions, and high storage and transportation costs Will limit the variety and market of IgY antibody drug products
Among them, oral administration has become the forefront of the development of international antibody drugs due to its convenience and safety, but the major problems faced are: The drug is easily degraded by gastric acid and digestive tract enzymes when it is taken directly orally; As a water-soluble macromolecule, the release of the drug is difficult to control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method and application of egg yolk antibody microcapsule preparation comprise the following steps:

[0031] (1) Immunization:

[0032] Choose healthy hens, feed them adaptively, and start immunization after one week of normal egg production. Inject rotavirus vaccine with Freund's adjuvant, 0.5mL each time, subcutaneously or intramuscularly, and immunize once every 7 days for a total of 4 times. Antibodies generally appear 14 days after the first immunization. Boost immunization once every 30 days, collect eggs, and store at 4°C;

[0033] (2) Antibody purification and separation preparation:

[0034] Disinfect the shell of the above egg with 75% alcohol, remove the shell and vitelline membrane in a sterile environment, take the yolk, and use the following method to prepare antibody preparations; method 1: pasteurize the egg yolk, freeze-dry, and make the egg yolk Egg yolk powder of virus IgY antibody, stored at 4°C; method two: purify egg yolk antibody p...

Embodiment 2

[0039] A preparation method and application of egg yolk antibody microcapsule preparation include the following steps:

[0040] (1) Immunization:

[0041] Choose healthy hens, feed them adaptively, and start immunization after one week of normal egg production. Inject inactivated rotavirus vaccine with Freund's adjuvant, 0.5 mL each time, subcutaneously or intramuscularly, and immunize once every 7 days for a total of 4 times. Antibodies generally appear 14 days after the first immunization. Boost immunization once every 30 days, collect eggs, and store at 4°C;

[0042] (2) Antibody purification and preparation:

[0043] The above-mentioned egg shells are sterilized with bromogeramine, the shells are removed, the yolk membrane is removed, and the yolk is taken, and the following method is used to prepare the antibody preparation; method 1: the yolk is pasteurized, freeze-dried, and the yolk powder containing rotavirus IgY antibody is made , stored at 4°C; Method 2: Purify e...

Embodiment 3

[0048] A preparation method and application of egg yolk antibody microcapsule preparation:

[0049] Oral administration of microcapsules made of rotavirus IgY antibody is a therapeutic drug for related diseases caused by rotavirus infection. IgY antibody microcapsules are used every day, and one month is a course of treatment. It is a treatment method for related diseases caused by rotavirus infection. Multiple courses of treatment may be performed as needed. Egg yolk powder containing rotavirus IgY antibody is added to food or made into health food, which can be used as an adjuvant therapy. Patients can take it at the same time as the conventional treatment drug regimen to enhance the resistance to rotavirus.

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PUM

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Abstract

The invention discloses a preparation method and an application of a yolk antibody microcapsule preparation. According to the invention, a vaccine prepared by using rotavirus is injected into a healthy laying female bird; after immunization, yolk is fetched, sterilized, separate-extracted, and purified, such that an anti-rotavirus IgY preparation is obtained; or IgY antibody preparations comprising various rotaviruses are mixed according to a certain ratio; an alginate solution with the mixed IgY antibody is dropped into a mixed solution of chitosan in a droplet manner, such that gel-state AP capsules are prepared, and the anti-rotavirus yolk antibody microcapsule preparation is obtained. The preparation is used in passive immunotherapy of rotavirus-infected patients, and is used in rotavirus prevention of high-risk populations.

Description

Technical field: [0001] The invention relates to the preparation and development of new biological drugs for treating and preventing rotavirus infection, especially the preparation method and application of anti-rotavirus egg yolk antibody microcapsule preparation. Background technique: [0002] Diarrheal disease is a high incidence of children in my country, accounting for about 12% to 24% of the total number of pediatric hospitalizations, and autumn diarrhea caused by rotavirus is the main disease from August to December. The application of anti-rotavirus chicken yolk immunoglobulin (IgY) in the treatment of rotavirus enteritis provides a new way for the protection and treatment of rotavirus enteritis by passive immunity. But so far, injection is still the only way of administration of this kind of drug in clinical application, and the outstanding problems are: The half-life of the drug in the blood is short, and it needs long-term frequent injection, which causes a great ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K9/52A61K47/36A61K39/395A61P31/14
Inventor 赵钢王智佳王国新
Owner TIANJIN JIMINGSHENG BIOLOGICAL TECH
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