Application of astragalus mongholicus water extract to biopesticides
A technology of water extract and Astragalus membranaceus, applied in the application field of Astragalus membranaceus water extract in pesticides, can solve the problems of unreported application and achieve the effects of wide sources, inhibition of plant pathogenic bacteria, and low pollution
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Embodiment 1
[0019] Test grasses: rape (Brassica rapa L. Chinensis Group), bermudagrass (Cynodon dactylon L.), duckweed (Lemna minor), provided by Hubei Academy of Agricultural Sciences.
[0020] Test method: When using pakchoi and bermudagrass as targets, the screening method is the agar medium surface coating method. Using a 96-well cell culture plate, add 300μL of water agar and about 10 seeds to each well, and then add 20μL of diluted drug solution. The initial screening drug solution concentration is 1.0mg / mL, 0.3mg / mL, and 0.1mg / mL. One week later, check the seed germination and plant growth. The re-screening concentration is adjusted according to the preliminary screening results.
[0021] Hydroponics is used when duckweed is the target. Using a 24-well cell culture plate, add 2 mL of duckweed culture nutrient solution to each hole, insert 3 duckweed leaves, and add 20 μL of diluted drug solution. The concentration of the drug solution at the initial screening was 1.0 mg / mL, and the g...
Embodiment 2
[0025] Test bacteria: Pythium ultimum, Botrytis cinerea, Septoria nodorum, Alternaria Solani, Fusariumculmorum, Rhizoctonia solani ), provided by Hubei Academy of Agricultural Sciences.
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