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Open-type plant tissue culture method

A plant tissue culture, open technology, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of high probability of culture contamination, unfavorable growth of culture, influence and other problems

Inactive Publication Date: 2013-03-27
SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant tissue culture is used to make medium, and the hot-melt agar nutrient solution is divided into culture bottles. The volume of the medium generally accounts for about one-fifth of the volume of the culture bottle, leaving a large space in the bottle. According to the published information, Look, in the existing open tissue culture, the addition of antibacterial agents to the medium can only partially inhibit the solid medium, and can do nothing to the microorganisms in the space in the bottle. The sediment attaches to the surface of the culture and reproduces, and the culture has a higher probability of contamination
[0004] Judging from the published data, the amount of antibacterial agent added to the medium may increase the osmotic pressure of the medium and have an adverse effect on the growth of the culture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment one, the application of unilateral antibacterial medium on the proliferation of Phalaenopsis protocorms

[0064] 1 / 2 macroelement MS medium, add 3 mg of hormone 6-BA, 30 g of sucrose, and 7 g of agar per liter, heat to melt completely, disconnect the heat source, constant volume, add various antibacterial agents in Table 1 to the medium, and adjust the pH to 5.8, without autoclaving, directly dispense into glass culture bottles, cover and seal within 5 minutes, inoculate Phalaenopsis protocorms in an open environment indoors, control medium without adding bacteriostatic agents, dispense into glass culture bottles, Routine autoclave sterilization, after cooling, inoculate protocorm-like plants by conventional methods, 15 bottles for each treatment, conventional culture, and observe the contamination of the culture bottles and the weight increase of protocorm-like plants after one month, see Table 1.

[0065] Table 1 Proliferation of Phalaenopsis protocorms on ...

Embodiment 2

[0067] Embodiment two, the application of unilateral antibacterial medium on carrot seed germination and long seedling

[0068] MS medium, sucrose 30g / L, agar 7g / L, heated to melt completely, cut off the heat source, constant volume, the medium was added with various antibacterial agents in the table below, adjust the pH to 5.8, without autoclaving, directly separate Put it into a glass culture bottle and seal it within 5 minutes. No bacteriostatic agent was added to the control medium, which was divided into glass culture bottles and sterilized by conventional high pressure, 10 bottles for each treatment.

[0069] De-stabbed carrot seeds are routinely sterilized with 75% alcohol in an instant, soaked in 0.1% mercury chloride for 10 minutes, inoculated in an open environment indoors, 30 grains per bottle, and cultivated conventionally. After one month, observe the number of bottles contaminated with the medium, the germination rate of the seeds, and the number of sprouts. Hei...

Embodiment 3

[0072] Embodiment three, the application of unilateral antibacterial medium on the long seedlings of cabbage seed germination

[0073] MS liquid medium, sucrose 30g / L, add various antibacterial agents, stir evenly, constant volume, adjust pH to 5.8, absorb a small amount, add filter paper glass culture bottle on the bottom pad, cover and seal within 5 minutes, without autoclaving bacteria. No antibacterial agent was added to the control medium, which was divided into glass culture bottles with filter paper at the bottom, and sterilized by conventional autoclaving, with 10 bottles for each treatment.

[0074] The seeds of Chinese cabbage were sterilized with 75% alcohol instantly, soaked in 0.1% mercuric acid for 10 minutes, added bacteriostatic medium and inoculated in an open environment indoors, and the control was inoculated with conventional methods, 40 grains per bottle, conventionally cultivated, and observed the medium after one month See Table 3 for the number of cont...

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Abstract

The invention discloses an open-type plant tissue culture method. A volatile plant essential oil bacteriostatic agent is added into a culture medium, a high-pressure disinfection process is saved, and inoculation is performed in an indoor open environment; and the culture material is cultured in an environment with the temperature and illumination conditions for plant tissue culture. According to the invention, the volatile bacteriostatic agent can disinfect the space in a bottle while disinfecting the culture medium, thus the culture grows in a relatively sterile environment, and the probability of the culture getting polluted is reduced; a small amount of bacteriostatic agent is added into the culture medium (the total amount is 2mul-2ml / L of culture medium), and the influence on the osmotic pressure of the culture medium is avoided; the components are non-toxic to human body; and the addition amount is small, and the irritant smell is avoided.

Description

technical field [0001] The invention relates to a plant tissue culture seedling raising technology in the field of agriculture and forestry. Background technique [0002] Plant tissue culture is a modern agricultural technology developed on the basis of plant physiology at the beginning of this century. After nearly a century of development. The method of asexual reproduction using plant tissue culture technology has made great progress. Compared with traditional plant breeding methods, this method is fast and efficient, and is not subject to seasonal restrictions. Sometimes it is combined with "detoxification" technology to achieve the effects of preventing poisonous degradation, enhancing vitality and better maintaining species. It is currently advocated Under the situation of efficient modern agricultural development mode, the production of high-quality seedlings through plant tissue culture technology has a very broad market prospect. Although its theoretical research...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘福平
Owner SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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