EB virus capsid antigen IgM antibody colloidal gold method detection reagent and preparation method thereof

Abstract

The present invention discloses an EB virus capsid antigen IgM antibody colloidal gold method detection reagent and a preparation method thereof. The reagent comprises a gold conjugate pad (3), a nitrocellulose reaction membrane (4), a sample pad (2), a rheumatoid factor treatment pad (1), water absorption paper (5) and a PVC backing (8), wherein a length of the rheumatoid factor treatment pad is approximately 1 / 2 of a length of the sample pad, the sample pad, the gold conjugate pad, the nitrocellulose reaction membrane and the water absorption paper are sequentially and mutually laminated and adhered on the PVC backing, the gold conjugate pad is coated with an anti-human IgM monoclonal antibody-colloidal gold conjugate, and positions of a quality control area (7) and a detection area (6) of the nitrocellulose reaction membrane are respectively coated with goat anti-mouse IgG antibody or rabbit anti-mouse IgG antibody and specific gene recombinant EB virus capsid antigen. According to the present invention, a production process is simple and easy to control, test results can be obtained within 25 minutes, self-detection can be achieved according to operations in a manufacturer's instruction, test results are not affected by the rheumatoid factor, sensitivity is high, specificity is good, and results are accurate and reliable.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to a reagent for detecting IgM antibody of EB virus capsid antigen by colloidal gold immunochromatography and a preparation method thereof. [0002] Background technique [0003] Epstein-Barr virus (EBV) is a human lymphocyte-tropic herpes virus, which is mainly transmitted through human saliva and can also be transmitted through blood transfusion. Virus. Epstein-Barr virus is closely related to infectious mononucleosis, Burkitt's lymphoma, Burkitt's lymphoma, nasopharyngeal carcinoma, Hodgkin's lymphoma, etc. It causes 1% of global cancers and accounts for all infectious cancers According to the International Agency for Research on Cancer's classification criteria for carcinogens, Epstein-Barr virus is included in the first group of carcinogens. [0004] After the human body is infected with Epstein-Barr virus, it can induce anti-nuclear antigen (EBna), early antigen (EA), capsid ...

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Problems solved by technology

However, due to the limited loading of protein in the unit microwell, this measure is difficult to achieve

[0008] Gold immunodiafiltration is a detection method that uses nitrocellulose membrane as a carrier and colloidal gold as a tracer. It has high specificity and high sensitivity, but the method is cumbersome and prone to confusion during clinical trials.

[0009] In addition, the above three methods are affected by rheumatoid factor-positive serum samples, which may easily cause false positives or missed detections. It is necessary to detect specific IgM antibodies after ultracentrifugation or immunosorbent treatment before turning negative. It is difficult to complete the detection in one experiment.

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