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Establishment of pigeonpea leaf herb finger print, and finger print thereof

A technology of fingerprints and pigeonpea leaves, which is applied in the field of quality control of traditional Chinese medicinal materials, can solve the problems of not being able to reflect the overall quality of medicinal materials, and achieve the effect of simple method, accurate authenticity, and good reproducibility

Inactive Publication Date: 2013-03-20
DONGGUAN MATHEMATICAL ENG ACAD OF CHINESE MEDICINE GUANGZHOU UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only relying on a single or a few chemical components is not enough to reflect the overall quality of medicinal materials

Method used

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  • Establishment of pigeonpea leaf herb finger print, and finger print thereof
  • Establishment of pigeonpea leaf herb finger print, and finger print thereof
  • Establishment of pigeonpea leaf herb finger print, and finger print thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1. Detection of the fingerprints of pigeon pea leaf medicinal material

[0057] 1. Instruments and reagents

[0058] 1.1 Instrument 1 / 100,000 electronic balance (Sartorius CP225D, German company Sartorius); Japan Shimadzu LC-20A (UV / Vis) high performance liquid chromatography: quaternary gradient pump (TLC-20AT), online degasser, Column thermostat (CTO-20AT), autosampler (SIL-20AT), detector (SPD-20AT); Shimadzu LC solution chromatography workstation; university liquid phase column: Dikma Diamonsil C 18(2) column (250× 4.6mm, 5μm).

[0059] 1.2 Reagents Vitexin reference substance; methanol (chromatographically pure), glacial acetic acid (analytical pure), ultrapure water.

[0060] 2. High performance liquid chromatography

[0061] 2.1 Chromatographic conditions: the chromatographic column is filled with octadecylsilane bonded silica gel; the mobile phase is a gradient eluent composed of methanol and 1.0% glacial acetic acid; the column temperature is 40°C; t...

Embodiment 2

[0069] Example 2. Detect the fingerprints of 12 batches of pigeonpea leaf medicinal materials

[0070] Take 12 batches of pigeonpea leaf medicinal material, detect according to the conditions of Example 1, and obtain the HPLC spectra of 12 batches of samples. Through the comparison of 12 batches of HPLC chromatograms, the similarity evaluation is carried out, and the characteristic common peaks are determined:

[0071] There are 11 common peaks in the fingerprint spectrum. Now the retention time of the spectrum, the average value of the peak area and the total peak area are summarized, as follows:

[0072] For peak No. 1, the average relative retention time RRT is 0.5832, the RSD is 0.14%, the average relative peak area RPA is 1.9761, and the RSD is 42.34%, accounting for 8.56% of the total peak area.

[0073] For peak No. 2, the average relative retention time RRT is 0.6455, the RSD is 0.11%, the average relative peak area RPA is 1.8675, and the RSD is 47.96%, accounting for...

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Abstract

The present invention provides establishment of a pigeonpea leaf herb finger print, and a finger print thereof, and relates to a quality control method for traditional Chinese medicine herbs, specifically to a method for establishing a HPLC finger print to detect a pigeonpea leaf herb extraction solution. The method comprises: (a) preparing a reference substance solution; (b) preparing a sample solution; (c) adopting the following chromatograph conditions, wherein a chromatographic column adopts octadecylsilane chemically bonded silica gel as a filler, gradient elution is performed, a mobile phase is a gradient elution solution comprising methanol and 1.0-3.0% glacial acetic acid, a column temperature is 20-40 DEG C, an ultraviolet detection wavelength is 220-300 nm, a flow rate is 0.5-1.2 mL.min<-1>, and a time is 100-140 min; and (d) determining, wherein a high performance liquid chromatography method is performed to determine the finger print. According to the present invention, quality of the pigeonpea leaf herb can be effectively characterized, herb quality monitoring can be easily achieved, the method has characteristics of simpleness, stability, high precision and good reproducibility, and authenticity of the herb can be quickly and accurately identified.

Description

technical field [0001] The invention relates to a quality control method of traditional Chinese medicinal materials, in particular to a construction method and a standard fingerprint of pigeonpea leaf medicinal material by high-performance liquid chromatography. Background technique [0002] Pigeon pea leaves are the leguminous plant pigeonpea ( Cajanus cajan (L.) Millsp) dry leaves and shoots. Pigeon pea is commonly used in English as Pigeonpea, and it is also known as soybean tree, bean curd tree, three-leaf bean, millennium bean, tree bean, Rong bean, Liu bean, flower bean, rice bean, etc. It is mainly distributed in Hainan, Yunnan, and South of the Yangtze River. Guangxi, Guizhou, Guangdong and Fujian, Hunan, Sichuan, Jiangxi, Zhejiang, Taiwan and other provinces are rich in resources. "Lu Chuan's Materia Medica" records that pigeonpea leaves are "flat, light, and slightly poisonous". "Chinese Medicine Sea" has further explained that pigeonpea leaf is "flat, light, sl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 黄松丁婕赖小平陈建南詹丽玲孙琳李婧王培培
Owner DONGGUAN MATHEMATICAL ENG ACAD OF CHINESE MEDICINE GUANGZHOU UNIV OF CHINESE MEDICINE
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