Novel phytosterol or/and phytostanol derivative preparation method

A technology for phytosterols and phytosterols, which is applied in the field of preparation of novel phytosterols or/and phytostanol derivatives, can solve the problem that the research on the modification of phytosterols or/and phytosterols is not comprehensive enough and the scope of application is small. And other problems, to achieve the effect of low reaction conditions, improve conversion rate, and broaden the scope of application

Inactive Publication Date: 2013-03-20
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] The purpose of the present invention is to develop a kind of novel phytosterol or / and the preparation method of phytostanol derivative for the shortcomings such as the modification research of current phytosterol or / and phytostanol is not comprehensive enough, the scope of application is small

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Weigh 12.4g of β-sitosterol, 7.9g of glutaric acid, and 0.4mL of catalyst pyridine into a reflux reaction device pre-added with 200mL of benzene and equipped with stirring and oil bath temperature control. The reaction temperature is controlled at 80°C, every 2 hours Sampling was carried out to detect the reaction progress. After 14 hours of reaction, the sample was taken out by rotary evaporation to remove the solvent to obtain the intermediate product β-sitosterol glutaric acid monoester.

Embodiment 2

[0052] Weigh 1.0 g of β-sitosterol glutaric acid monoester and 0.7 g of glucose in a 50 mL reaction bottle, add 10% of the total mass of β-sitosterol glutaric acid monoester and glucose to the immobilized lipase derived from thermophilic fungi , add 25mL acetone as a solvent, 1.0g molecular sieves, airtight, place in a water bath shaker, control the rotation speed 150rpm, the reaction temperature is 45°C, take samples every 12 hours to detect the reaction process, and react for 72 hours; after high performance liquid chromatography analysis, the reaction solution The purity of β-sitosterol glucoglutarate in the medium is 65%; the water-soluble β-sitosterol derivative-sitosterol glucoglutarate with a purity of more than 95% is obtained through separation and purification by column chromatography.

Embodiment 3

[0054] Weigh 12.5g of sitostanol, 10.4g of suberic acid, and 0.5g of copper dodecyl sulfate as a catalyst, and add it to a reflux reaction device with 200mL of toluene added in advance and equipped with stirring and oil bath temperature control, and the reaction temperature is controlled at 110°C , Sampling every 2 hours to detect the reaction process, reacting for 12 hours, taking out the sample and rotary evaporating to remove the solvent to obtain the intermediate product sitostanol suberic acid monoester.

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PUM

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Abstract

The present invention relates to a novel phytosterol or / and phytostanol derivative preparation method, and belongs to the technical field of food, medicine, chemical industry, cosmetics and the like. In the prior art, modification researches of phytosterol or / and phytostanol are only limited in liposolubility improvement, and an application range is limited. A purpose of the present invention is to improve water solubility of phytosterol or / and phytostanol. The preparation method comprises the following steps that: a) a certain amount of phytosterol or / and phytostanol and a polybasic organic acid or anhydride are subjected to a reaction under a catalyst effect to generate an intermediate product phytosterol or / and phytostanol polybasic acid monoester; and b) the intermediate product and a polyhydroxy compound are subjected to a reaction under a catalysis effect of a biocatalyst catalyst to generate a water-soluble phytosterol or / and phytostanol derivative, and the obtained water-soluble phytosterol or / and phytostanol derivative is subjected to column chromatography separation purification to obtain the novel phytosterol or / and phytostanol derivative.

Description

technical field [0001] The invention relates to a method for preparing novel phytosterols or / and phytostanol derivatives, in particular to a method for enzymatically synthesizing water-soluble phytosterols or / and phytostanol derivatives in an organic phase. The application and development of this product involves technical fields such as food, medicine, chemical industry and cosmetics. Background technique [0002] Phytosterols are a class of steroidal compounds with cyclopentaneperhydrophenanthrene as the main skeleton, mainly found in nuts, seeds, grains, etc. Phytosterols are generally extracted from the deodorized distillate during vegetable oil refining, and large-scale industrial production has been carried out in China at present. Phytosterols mainly include β-sitosterol, stigmasterol, brassicasterol, and campesterol, among which β-sitosterol is the main one; the double bonds in phytosterols are hydrogenated and saturated, which are called phytostanols, mainly includ...

Claims

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Application Information

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IPC IPC(8): C12P33/00
Inventor 冯骉何文森贾承胜张晓鸣
Owner JIANGNAN UNIV
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