Direct-throwing type sour dough fermenting agent as well as preparation method and application thereof
A direct-injection, starter technology, applied in the field of food processing
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Embodiment 1
[0095] Embodiment 1: the preparation of direct throw type plant lactic acid bacteria sourdough starter
[0096] The implementation steps of this embodiment are as follows:
[0097] A. Activation of Lactobacillus plantarum
[0098] Draw 200 μl of plant lactic acid bacteria CCFM8661 bacteria liquid from the glycerol bacteria preservation tube, inoculate it into 800ml MRS broth medium, and then carry out activation culture at a temperature of 36°C for 24 hours, repeat the above activation operation 4 times, and obtain an activated bacteria liquid; The MRS broth culture medium composition described is as follows: 500ml distilled water, 5g peptone, 5g beef extract, 2.5g yeast extract, 2.5g sodium acetate, 10g glucose, 0.05g MgSO 4 .7H 2 0.0.025g MnSO 4 .H 2 0, 1g diammonium citrate, 1g K 2 HP0 4 With 0.5ml Tween, pH 6.2~6.4.
[0099] B, the preparation of direct-throwing type plant lactobacillus dough starter
[0100] The activated bacterial liquid obtained in step A was ce...
Embodiment 2
[0103] Embodiment 2: the preparation of direct throw type plant lactic acid bacteria sourdough starter
[0104] The implementation steps of this embodiment are as follows:
[0105] A. Activation of Lactobacillus plantarum
[0106] Draw 200 μ l of plant lactic acid bacteria CCFM8661 bacteria liquid from the glycerol bacteria preservation tube, inoculate it into 1200 ml of the same MRS broth medium as in Example 1, then carry out activation culture at a temperature of 38 ° C for 18 h, repeat the above activation operation 2 times, and obtain a An activated bacterial liquid.
[0107] B, the preparation of direct-throwing type plant lactobacillus dough starter
[0108] The activated bacterial liquid obtained in step A was centrifuged at a high speed for 3min under the condition of 7000g, and the separated bacterial cells were washed 4 times with the same aseptic phosphate buffer solution as in Example 1, thus obtaining a concentration of 8 × 10 10 cfu / ml active sludge.
[0109...
Embodiment 3
[0115] Embodiment 3: the preparation of single-plant Lactobacillus fermented type direct throw type sourdough starter
[0116] The implementation steps of this embodiment are as follows:
[0117] A. Activation of Lactobacillus plantarum
[0118] Draw 200 μ l of plant lactic acid bacteria CCFM8661 bacteria liquid from the glycerol bacteria preservation tube, inoculate it into 1200 ml of the same MRS broth medium as in Example 1, then carry out activation culture at a temperature of 38 ° C for 18 h, repeat the above activation operation 2 times, and obtain a An activated bacterial liquid.
[0119] B, the preparation of direct-throwing type plant lactobacillus dough starter
[0120] The activated bacterial liquid obtained in step A was centrifuged at a high speed for 3min under the condition of 7000g, and the separated bacterial cells were washed 4 times with the same aseptic phosphate buffer solution as in Example 1, thus obtaining a concentration of 8 × 10 10 cfu / ml active s...
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