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Method for analyzing and detecting inulin in infant formula

A technology for infant formula and milk powder, which is applied in the field of analytical chemistry, can solve problems such as the difficulty in improving the accuracy of test results and the indistinguishability of inulin analysis and testing methods, and achieve high accuracy results

Inactive Publication Date: 2013-03-06
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is that the existing inulin analysis test method cannot distinguish the limitations of inulin and fructo-oligosaccharide in infant milk powder, which leads to the defect that the accuracy of the test result is difficult to improve. Fructose-oligosaccharide interference, an analytical method for the detection of inulin in infant formula

Method used

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  • Method for analyzing and detecting inulin in infant formula
  • Method for analyzing and detecting inulin in infant formula
  • Method for analyzing and detecting inulin in infant formula

Examples

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Embodiment 1

[0031](1) Ultrafiltration treatment of infant formula milk powder samples

[0032] Weigh 2g of milk powder into a 50ml beaker, add 10ml of 60°C hot water into the beaker, and stir well with a glass rod. Then, add the milk powder solution into 1.5ml centrifuge tubes with a pipette gun and centrifuge for 5 minutes (conditions are 13000 rpm, 4°C). After centrifugation, take out the supernatant of the centrifuge tube, perform ultrafiltration (3700g×30min) with a molecular weight cut-off ultrafiltration tube of 10KD, and take the filtrate after the ultrafiltration.

[0033] (2) Determination method of fructooligosaccharides

[0034] 1. Chromatographic analysis of fructooligosaccharide content

[0035] Select five concentrations of mixed standard solutions, inject three times for each concentration, take the average value of the three times, and perform linear regression with the response ratio to the concentration ratio to calculate the standard curve. According to this standard...

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Abstract

The invention provides a method for analyzing and detecting inulin in infant formula. The method includes: (1) removing biomacromolecules in infant formula by means of ultrafiltration; and (2) testing content of fructo-oligosaccharide and total levan in the infant formula: determining and calculating the kestose percentage composition i3, the nystose percentage composition i4, fructofuranosylnystose percentage composition i5, lactose percentage composition Las1 and sucrose percentage content S1 in the infant formula by means of efficient liquid chromatography; using inulase for enzymolysis of sample solution, performing centrifugal ultrafiltration, and determining and calculating fructose percentage composition Ft and glucose percentage composition Gt in the sample subjected to enzymolysis and the percentage composition Las2 of the residue lactose not subjected to enzymolysis by means of efficient liquid chromatography; and conversing the inulin percentage composition i6 according to a formula a, wherein the conversion coefficient k is 0.9. The method is extremely high in accuracy and is capable of successfully detecting the inulin content in the infant formula.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a method for analyzing and detecting inulin in formula milk powder for infants and young children. Background technique [0002] In the field of food safety testing, food additives have always been the focus of attention. This is because there are usually many types of food additives, and there are often problems of irregular use. In recent years, with the improvement of people's living standards and the large intake of high-fat, high-protein, and refined foods, there are more and more people with "three highs"; and dietary fiber has unique effects in improving nutritional balance, so it is often used as food Additives are added to food. Inulin (a mixture of fructans with a degree of polymerization of 2-60, usually the average degree of polymerization of fructans in commercial inulin is 10-30) is a kind of natural dietary fiber, which exists in almost all plants , which is ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/74
Inventor 曹旭妮徐晓婧夏琨
Owner EAST CHINA UNIV OF SCI & TECH
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