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Rapid propagation method of nothapodytes pittosporoides

A rapid, explant-based technique applied in the field of bioengineering to achieve the effects of simple equipment, less floor space, and low production costs

Inactive Publication Date: 2013-03-06
向华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there have been no reports on the success of Mabi wood tissue culture rapid propagation at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Material: Mabi wood with axillary bud stems as explants

[0026] 1. Selection and sterilization of explants: Young tissues such as fresh shoots and stems of Mabi wood collected from Cili, Hunan, as culture materials, washed with detergent and tap water, rinsed under running water for 15 minutes, Place it in 75% alcohol for 10-30 seconds, then disinfect it with 0.1% mercuric chloride for 12 minutes, rinse it with sterile water, cut off the discolored part of the explant and set it aside for later use.

[0027] 2. Germination of buds: Inoculate the sterilized buds on Ms+6-BA1.2mg / L+IBA0.2mg / L medium, the pH value is 5.8, the culture temperature is 25-27°C, and the light is on every day 12 hours, the light intensity is 1000LX; 10 days after inoculation, the buds germinate, and the seedlings with leaves are formed in about 30 days.

[0028]3. Cluster bud induction and proliferation culture: cut the seedlings and transfer them to Ms+6-BA1.2mg / L+IBA0.4mg / L medium until clust...

Embodiment 2

[0032] Material: Mabi wood with axillary bud stems as explants

[0033] 1. Selection and sterilization of explants: Young tissues such as fresh shoots and stems of Mabi wood collected from Cili, Hunan, as culture materials, washed with detergent and tap water, rinsed under running water for 10 minutes, Place it in 75% alcohol for 10-30 seconds, then disinfect it with 0.1% mercuric chloride for 15 minutes, rinse it with sterile water, cut off the discolored part of the explant and set it aside for later use.

[0034] 2. Germination of buds: Inoculate the sterilized buds on Ms+6-BA1.2mg / L+IBA0.2mg / L medium, the pH value is 5.8, the culture temperature is 25-27°C, and the light is on every day 16 hours, the light intensity is 1000LX; 15 days after inoculation, the buds germinate, and the seedlings with leaves are formed in about 30 days.

[0035] 3. Cluster bud induction and proliferation culture: cut the seedlings and transfer them to Ms+6-BA1.2mg / L+IBA0.4mg / L medium until clus...

Embodiment 3

[0039] Material: Mabi wood with axillary bud stems as explants

[0040] 1. Selection and sterilization of explants: young tissues such as fresh buds and stems of Mabi wood collected from Sangzhi, Hunan, as culture materials, washed with detergent and tap water, rinsed under running water for 30 minutes, Place it in 75% alcohol for 10-30 seconds, then disinfect it with 0.1% mercuric chloride for 13 minutes, rinse it with sterile water, cut off the discolored part of the explant and set it aside for later use.

[0041] 2. Germination of buds: Inoculate the sterilized buds on Ms+6-BA1.2mg / L+IBA0.2mg / L medium, the pH value is 5.8, the culture temperature is 25-27°C, and the light is on every day 16 hours, the light intensity is 1000LX; 12 days after inoculation, the buds germinate, and the seedlings with leaves are formed in about 30 days.

[0042] 3. Cluster bud induction and proliferation culture: cut off the seedlings and transfer to Ms+6-BA1.2mg / L+IB0.4mg / L medium until clust...

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Abstract

The invention relates to a rapid propagation method of nothapodytes pittosporoides, and the method is characterized in that tissues such as tender shoot sections are cultivated on a murashige and skoog (MS) culture medium, a tender shoot stem section with one to two sprouts is cut as explants, the explants are grafted in MS+6-butyl acrylate (BA) 1.2mg / L+ indole butyric acid (IBA)0.2mg / L culture medium after being sterilized, the bud is germinated in 10 to 15 days after the grafting, seedlings with leaves are formed in 30 days, the seedlings are cut off and transferred onto the MS+6-BA1.2mg / L+IBA0.4mg / L culture medium to keep the high-speed increase, and the propagation coefficient is more than 5. The MS+6-BA0.5mg / L+ naphthyl acetic acid (NAA)1.5mg / L is selected as rooting culture medium, the rooting rate reaches more than 95 percent, and a complete plant can be formed in 30 days. Test-tube plantlets are transplanted in sandy soil, attention is paid on the moisture preservation, the transplanting survival rate can reach up to 93 percent after the test-tube plantlets are transplanted for 45 days, and a high-frequency stable renewable system is established. The method has the advantages of good stability, simplicity and convenience in operation, fast propagation speed, low production cost, industrialization and the like.

Description

technical field [0001] The invention relates to the field of bioengineering, and relates to plant tissue culture technology, in particular to a method for tissue culture of mabi wood. Background technique [0002] Plants are a natural treasure house of medicines. People have a long history of using medicinal plants. About 75% of the world's population uses plants as a source of medicine for treating and preventing diseases (Xing Jianmin, 2001). Humans have discovered many drugs with high physiological activity from plants, and the drugs derived from plants account for more than 25% of the total amount of drugs (Zheng Guangzhi, 1987). Our country's traditional Chinese herbal medicine has a history of thousands of years, and it is still widely used in our country and many countries and regions. However, because the traditional method of obtaining Chinese herbal medicine is at the cost of collecting and consuming a large amount of wild plant resources, when the amount of colle...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 向华
Owner 向华
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