Method for quickly transforming cytoplasmic male sterile line of brassica vegetables

Abstract

The invention provides a method for quickly transforming a cytoplasmic male sterile line of brassica vegetables, which comprises the steps of: using a brassica breeding material with the cytoplasmic male sterile line as a female parent, and using pure brassica breeding material with genome type different from that of the female parent as a male parent for interspecies cross, obtaining interspecies cross F1 plant by an embryo rescue technology, then backcrossing with the male parent continuously, and quickly obtaining the cytoplasmic male sterile line through field selection and chromosome identification. The method has the beneficial effects that: 1) the breeding process of the cytoplasmic male sterile line of brassica vegetables is accelerated by interspecies cross characteristics of three genomes of cabbage, leaf mustard and wild cabbage in the brassica vegetables; 2) flowering cabbage or wild cabbage is selected as the source of cytoplasmic male sterile resource, so that a quick transforming method system which covers the cytoplasmic male sterile lines of all genome types of the brassica vegetables is formed; and 3) flowering cabbage or wild cabbage directly bolts and blossoms without vernalization, so that the sterile line transforming time is shortened.

Description

technical field [0001] The invention relates to the breeding technology of cruciferous vegetables, in particular to a method for rapidly transferring cytoplasmic male sterile lines of Brassica vegetables. Background technique [0002] Brassicaceae Brassica vegetables are rich in varieties, including Chinese cabbage (Brassica rapa, chromosome group AA, 2n=20) (such as Chinese cabbage, rapeseed, Chinese cabbage, cabbage, etc.), cabbage (Brassica oleracea, chromosome group CC, 2n=18) (such as cabbage, cauliflower, broccoli, etc.), mustard greens (Brassica juncea, chromosome set AABB, 2n=36) (such as leaf mustard, root mustard, stem mustard, etc.), which are currently the most widely distributed in my country. A large class of vegetable crops with the largest cultivation area and the most common edible population occupies a pivotal position in my country's vegetable production. [0003] Brassicaceae Brassica vegetables have obvious heterosis. At present, most of the excellent var...

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Problems solved by technology

[0004] At present, in the breeding work of Brassica vegetables, using the conventional backcross transfer method, it takes at least 7-8 generations from the transfer to obtain the cytoplasmic male sterile source to the backcross purification to obtain a genetically stable homozygous sterile line. Therefore, constructing a rapid cytoplasmic male sterile transfection and purification system for Brassica vegetable crops, shortening the breeding process, improving the seed production quality of the first generation of hybrids, and making cytoplasmic male sterile lines widely used in Brassica vegetable breeding is of great significance

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