Method for quickly transforming cytoplasmic male sterile line of brassica vegetables
A male sterile line and male sterility technology, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of long breeding cycle and shorten the breeding process, and achieve the effect of accelerating the breeding process.
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Embodiment 1
[0058] Example 1 Breeding of Chinese cabbage cytoplasmic male sterile line——Ogura CMSR 3 Forty-Nine Caixin CMS
[0059] Adopt the method of the present invention, according to figure 2 Pedigree diagram shown for breeding Ogura CMSR 3 Forty-nine Caixin CMS:
[0060] 1) Ogura CMSR with cytoplasmic male sterility 3 The improved radish cytoplasmic sterile material CMSR 3 625 (chromosomal type is CC) as the female parent and Sijiu Caixin (chromosome type is AA, 2n=20) as the male parent. Interspecific crosses were carried out in the greenhouse of the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences in the spring of 2000. Through Obtaining Cytoplasmic Male Sterility Ogura CMSR Using Embryo Rescue Technology 3 interspecific hybrid F 1 plants.
[0061] The above-mentioned embryo rescue techniques are specifically:
[0062] a) During interspecific hybridization, the female and male parents are pollinated at the artificial bud stage. The m...
Embodiment 2
[0076] Example 2 Breeding Chinese cabbage cytoplasmic male sterile line——G2B male sterile line
[0077] Adopt the method of the present invention, according to Figure 6 The pedigree diagram shown is the selection of G2B male sterile line:
[0078] Breeding Ogura CMSR by traditional methods 3 104 white-flowered kale male sterile line: use 104 white-flowered kale as the male parent, and use the improved radish cytoplasmic sterile material CMSR3 After crossing 625 as the female parent and backcrossing 104 white-flowered kale as the male parent for 8 consecutive generations, a genetically stable OguraCMSR was obtained 3 104 white-flowered Chinese kale male sterile line with cytoplasmic male sterile source Ogura CMSR 3 , is an early-maturing material with a short growth cycle, bolting and flowering without vernalization, and can be planted at any time, providing a sterile source to transfer flower organs.
[0079] 1) Ogura CMSR with cytoplasmic male sterility 3 Ogura CMSR 3 1...
Embodiment 3
[0095] Example 3 Breeding mustard cytoplasmic male sterile line——No. 11 spherical heart mustard male sterile line
[0096] Adopt the method of the present invention, according to Figure 10 The pedigree shown in the figure shows the breeding of No. 11 Spherical Brassica male sterile line:
[0097] Breeding Ogura CMSR by traditional methods 3 104 white-flowered kale male sterile line: use 104 white-flowered kale as the male parent, and use the improved radish cytoplasmic sterile material CMSR 3 After crossing 625 as the female parent and backcrossing 104 white-flowered kale as the male parent for 8 consecutive generations, a genetically stable OguraCMSR was obtained 3 104 white-flowered Chinese kale male sterile line with cytoplasmic male sterile source Ogura CMSR 3 , is an early-maturing material with a short growth cycle, bolting and flowering without vernalization, and can be planted at any time, providing a sterile source to transfer flower organs.
[0098] 1) Ogura CMS...
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