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Original celastrus angulatus medicine as well as preparation method and mass detection method thereof

A quality detection method, the technology of paretin, which is applied in the field of botanical pesticides and can solve the problems of fingerprint detection that have not been reported.

Inactive Publication Date: 2014-11-05
河南省科高植物天然产物开发工程技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the products of pharegenin extract or the original drug and its fingerprint detection

Method used

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  • Original celastrus angulatus medicine as well as preparation method and mass detection method thereof
  • Original celastrus angulatus medicine as well as preparation method and mass detection method thereof
  • Original celastrus angulatus medicine as well as preparation method and mass detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0127] ④ Preparation of the test solution

[0128] Accurately weigh 200mg of 15% T12 phasanthalin or 2% phasanthalin water suspension in a 100mL volumetric flask, add an appropriate amount of methanol to dissolve, dilute to the mark after ultrasonic oscillation, and shake well to obtain 15% photericin Rattan original drug or 2% phaelopedin aqueous suspension for the test solution.

[0129] ⑤Measurement of precision

[0130] The test solution was repeatedly injected 5 times, the relative standard deviation of the peak area was RSD=1.02%, and the relative standard deviation of the retention time was RSD=0.45%.

[0131] ⑥ Determination of the test product

[0132] Draw each test product solution, inject sample, measure its peak area, and obtain the content of picrocetin B.

[0133] ⑦Determination of recovery rate

[0134] Using the standard addition method, 100 μg / mL was added to the test product of phareidin original drug, and the average recovery rate was 103.5%.

[0135] ...

Embodiment 1

[0264] (1) Preparation method of 15% T12 picrodermin original drug A

[0265] After sieving, the vine root or / and skin (24 mesh) of Shaanxi Baoji bitter bark that meets the requirements is sequentially added with ethanol with a volume of 3︰2︰2 times the amount (mass ratio) of 95% (mass ratio), and refluxed at 80°C for 2 hours (extraction 3 times, 2h each time), filter, combine the three filtrates, and concentrate under reduced pressure to a filtrate with a density of 1.08. Take this filtrate, add 1 times its volume of precipitant (10% ethanol by mass ratio), precipitate for 12 hours, and centrifuge to obtain the precipitate, that is, 15% T12 phalaetanin original drug A.

[0266]

[0267] (2) Quality testing method of 15%T12 picratine original drug A

[0268] ①Preparation of characteristic extracts

[0269] Take 1 g of 15% T12 phareidin original drug A, add 6 times the volume (6 mL) of chloroform, reflux extraction at 80 ° C for 40 min, filter and concentrate under reduced...

Embodiment 2

[0285] (1) Preparation method of 15% T12 picrodermin original drug A

[0286] Other conditions are the same as in Example 1, except that water with twice the volume of the filtrate is used as a precipitant, and the effect is similar to that of Example 1.

[0287]

[0288] (2) Quality testing method of 15%T12 picratine original drug A

[0289] ①Preparation of characteristic extract-same as Example 1.

[0290] ②Feature extraction IGD carbon NMR fingerprint detection

[0291] With embodiment 1.

[0292] ③Fingerprint analysis of IGD carbon NMR spectra of feature extracts

[0293] 1) Identification

[0294] In the IGD carbon NMR fingerprint of the characteristic extract of 15% T12 pharethin original drug A, the characteristic signal of 4-OH-β-dihydrofuran sesquiterpene polyol esters is clearly displayed. According to the chemical shift of its parent nucleus signal and its C-15 characteristic peak signal, 12 4-OH-β-dihydroagarfuran sesquiterpene polyol ester compounds picroc...

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Abstract

The invention relates to original celastrus angulatus medicine, a water suspension agent of the original celastrus angulatus medicine, as well as a preparation method and a mass detection method of the original celastrus angulatus medicine and the water suspension agent. The original celastrus angulatus medicine comprises the following active ingredients by mass percentage: 2.5%-4.0% of angulatus A, 3.0%-5.0% of angulatus B, 1.5%-3.0% of angulatus C, angulatus F and angulatus P, 1.5%-3.0% of angulatus G, 0.05%-1.00% of celastrus angulatus IV, 3.5%-5.5% of angulatus A, angulatus E and celastrus angulatus XIX, and 1.0%-2.0% of angulatus H. The original celastrus angulatus medicine is prepared by a precipitant precipitating method. The mass detection method adopts an IGD (inverted gated decoupling) carbon nuclear magnetic resonance coupling fingerprint technology. The content of the original celastrus angulatus medicine is increased greatly, and the mass detection method is higher in accuracy, stability, repeatability and practicability.

Description

technical field [0001] The invention belongs to the technical field of botanical pesticides, and in particular relates to a pharodermatine original drug and its water suspension, as well as its preparation method and quality detection method. Background technique [0002] Celastrus angulatus MaXim (Celastrus angulatus MaXim), also known as bitter bark and vegetable medicine, belongs to the genus Celastrus angulataceae. It is widely distributed in the provinces and regions of the Yangtze River and Yellow River Basin in my country. Its root bark and leaves have been used as insecticides by folks, and can also be used as medicine to treat sores and swellings. It has the function of clearing away heat and detoxification, and the root bark has the best insecticidal effect [Northwest Institute of Botany, Chinese Academy of Sciences. Qinling Plants Chi 1981, Beijing: Science Press]. The insecticidal active ingredients of Phyllostachys chinensis are a series of isolated 4-OH-β-dih...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/12A01N25/04A01P7/04G01N24/08G01N30/02
Inventor 张海艳赵天增董建军刘雨晴郭唯于立芹范毅常霞王伟王韬魏悦
Owner 河南省科高植物天然产物开发工程技术有限公司
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