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Method for studying synergistic effect of surfactant producing bacteria on petroleum degradation

A surfactant and synergistic technology, applied in the field of synergistic effect of surfactant producing bacteria on petroleum degradation, can solve the problems of high cost, secondary pollution, unsatisfactory effect, etc.

Inactive Publication Date: 2013-01-30
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods can have secondary pollution to the environment, and are expensive, and the effect received in practical application is not ideal

Method used

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  • Method for studying synergistic effect of surfactant producing bacteria on petroleum degradation
  • Method for studying synergistic effect of surfactant producing bacteria on petroleum degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Cultivation method for degrading petroleum pollutants by using mixed bacterial flora

[0028] The formula is sodium nitrate 0.5g / L, potassium dihydrogen phosphate 0.5g / L, potassium dihydrogen phosphate 0.5g / L, ammonium sulfate 2g / L, NaCl3g / L, magnesium sulfate 0.5g / L, trace elements 10mL / L , after adjusting the pH to 6.5 in the inorganic salt medium, after adding 5mL of crude oil and the initial OD 600 Acinetobacter calcium acetate 061021B with a concentration of 0.01 was placed in a constant temperature incubator at 30°C and 200rpm to carry out the degradation experiment of petroleum pollutants. After 10 days of degradation, use n-hexane to extract the undegraded crude oil in the medium, and use the specific gravity method to compare The change of oil weight before and after degradation, the experimental results show that the degradation rate of crude oil is 61.27%.

Embodiment 2

[0030] Cultivation method for degrading petroleum pollutants by using mixed bacterial flora

[0031] The formula is sodium nitrate 1g / L, potassium dihydrogen phosphate 1g / L, potassium dihydrogen phosphate 1g / L, ammonium sulfate 3g / L, NaCl 5g / L, magnesium sulfate 1g / L, trace elements 10mL / L, adjust the pH to After adding 5mL of crude oil to the inorganic salt medium after 7.0 and the initial OD 600 Acinetobacter calcium acetate 061021B with a concentration of 0.01 was placed in a constant temperature incubator at 30°C and 200rpm to carry out the degradation experiment of petroleum pollutants. After 10 days of degradation, use n-hexane to extract the undegraded crude oil in the medium, and use the specific gravity method to compare The change of oil weight before and after degradation, the experimental results show that the degradation rate of crude oil is 74.32%.

Embodiment 3

[0033] Cultivation method for degrading petroleum pollutants by using mixed bacterial flora

[0034] The formula is sodium nitrate 1.5g / L, potassium dihydrogen phosphate 1.5g / L, potassium dihydrogen phosphate 1.5g / L, ammonium sulfate 5g / L, NaCl7g / L, magnesium sulfate 1.5g / L, trace elements 10mL / L , after adjusting the pH to 7.5 in the inorganic salt medium, after adding 5mL of crude oil and the initial OD 600 Acinetobacter calcium acetate 061021B with a concentration of 0.01 was placed in a constant temperature incubator at 30°C and 200rpm to carry out the degradation experiment of petroleum pollutants. After 10 days of degradation, use n-hexane to extract the undegraded crude oil in the medium, and use the specific gravity method to compare The change of oil weight before and after degradation, the experimental results show that the degradation rate of crude oil is 54.91%.

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Abstract

The invention relates to a method for studying synergistic effect of surfactant producing bacteria on petroleum degradation. According to the method, a mineral culture medium for use in degradation experiments is prepared; and Pseudomonas aeruginosa GIMT1.074 is capable of producing surfactant in a mineral culture medium with glycerol as the only carbon source. Pseudomonas aeruginosa GIMT1.074 and acinetobacter calcoaceticus 061021B are inoculated with the initial OD600 being 0.01, and three groups of parallel tests are carried out respectively; according to the distinct phenotype of pseudomonas aeruginosa GIMT1.074 and acinetobacter calcoaceticus 061021B in a panel, the number of each kind of bacteria at corresponding moment is obtained by a plate count method, and the synergistic effect of pseudomonas aeruginosa GIMT1.074 and acinetobacter calcoaceticus 061021B in petroleum degradation is studied. The degradation rate of petroleum pollutants is improved by 12.83% due to the synergistic effect of the two strains.

Description

technical field [0001] The invention relates to the technical field of petroleum pollution, in particular to a method for researching the synergistic effect of surfactant-producing bacteria on petroleum degradation. Background technique [0002] Petroleum is a kind of hydrocarbons (normal alkanes, branched chain alkanes, cycloalkanes, aromatic hydrocarbons, polycyclic aromatic hydrocarbons, etc.) and a small amount of non-hydrocarbon compounds (sulfides, nitrides, alkane acids, etc.) , nickel, etc.), the environmental pollution caused during storage, transportation and processing has threatened the survival and development of humans and other organisms. Understanding the biodegradation and transformation laws of petroleum pollutants in nature, studying the principles and methods of microbial degradation of petroleum pollutants, separating, screening and cultivating petroleum-degrading bacteria, eliminating and reducing the retention of petroleum in the environment will help ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12R1/385C12R1/01
Inventor 贾晓强周征西闻建平
Owner TIANJIN UNIV
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