Deafness susceptive gene joint detection kit

Abstract

The invention discloses a deafness susceptive gene joint detection kit which comprises (1) a gene chip and (2) various primers, wherein the gene chip is provided with (i) nucleotide sequence probes and (ii) a DNA (deoxyribonucleic acid) sequence marked with biotin sites; the nucleotide sequence probes are at least one of sequences shown as SEQ ID Nos:1-26 and one of sequences complementary with the sequences shown as SEQ ID Nos:1-26; the DNA sequence marked with the biotin sites is as shown in SEQ ID No.27; and the primers are respectively shown as SEQ ID No.28-29, SEQ ID No.30-31, SEQ ID No.32-37 and SEQ ID No.38-43. The kit disclosed by the invention can realize synchronous joint detection, enhance the detection specificity, reduce the cost and shorten the detection time.

Description

technical field [0001] The invention relates to the field of genes, in particular to a combined detection kit for deafness susceptibility genes. Background technique [0002] Deafness is a common congenital disease that seriously affects the quality of human life. It can be caused by a single gene mutation or a compound mutation of different genes, or by environmental factors (such as medical factors, environmental exposure, trauma, drugs, etc.) or genetic and environmental factors. Both work together. Worldwide, there is one child with congenital deafness in every 1,000 newborns, and 50% of the children's deafness is related to genetic factors. According to statistics from the website of the China Disabled Persons' Federation, 21 million of China's 60 million disabled people are hearing disabled. Among those with hearing and speech disabilities, there are 800,000 deaf children under the age of 7, and the number of deaf children is increasing at an annual rate of 30,000. ...

AI Technical Summary

Problems solved by technology

However, due to the complex production process of the gene chip technology, the high precision required, it is difficult for ordinary laboratories to meet the requirements, and the supporting analysis software is not uniform, etc., and because it has just entered industrialization, the price is relatively expensive. These shortcomings make the application of this technology subject to certain restrictions. Restrictions, unable to meet the requirements of clinical rapid detection

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