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Heavy metal resistant strain for degrading polycyclic aromatic hydrocarbons (PAHs) and application thereof in combined contaminated soil remediation

A technology for polycyclic aromatic hydrocarbons and complex pollution, applied in the fields of environmental engineering and microbial engineering, to achieve the effects of rapid degradation ability, wide range of degradation substrates, and high degradation rate

Active Publication Date: 2013-01-09
深圳研源环境控股有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, there is a lack of a polycyclic aromatic hydrocarbon degrading bacteria that can tolerate various heavy metal environments in the prior art.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The separation and screening method of Tzyx2 provided by the invention.

[0040] Collect surface soil samples 5-20cm below the surface from the solid waste dismantling site, and use them as bacteria sources for natural enrichment culture. Glucose 2g, tryptone 4g, beef extract 4g, MgSO 4 Add 0.8g into 400mL of distilled water, sterilize the prepared culture solution at 121°C for 20 minutes, cultivate on this medium for three days, then prepare inorganic salt basic medium, add naphthalene, phenanthrene, fluoranthene , pyrene, benzoanthracene, polycyclic aromatic hydrocarbon compounds of benzopyrene, then add heavy metal ions, after being prepared into a solution, then add distilled water to settle to 500mL, add naphthalene, phenanthrene, fluoranthene, pyrene, benzoanthracene, The amount of benzopyrene will make the final concentration of naphthalene, phenanthrene, fluoranthene, pyrene, benzoanthracene, and benzopyrene in the prepared culture solution be 200 mg / mL, and th...

Embodiment 2

[0043] Tzyx2 degrades polycyclic aromatic hydrocarbons in complex polluted soils.

[0044] Select 100g of composite polluted soil with pyrene, benzanthracene, and benzopyrene concentrations of 200 mg / kg each, copper sulfate, zinc chloride, and lead nitrate concentrations of 600 mg / kg each, and use 2.0% (mass percentage) of Tzyx2 with Mix the soil evenly, add water to keep the moisture content of the soil at 15%, put it into a constant temperature oscillator, control the culture temperature at a constant temperature of 25°C, and shake the culture for 120 hours to restore the complex polluted soil. After soil, the degradation rates of pyrene, benzanthracene and benzopyrene are all above 90%.

[0045] Utilizing the method of the present invention to remediate compound polluted soil has a good remediation effect on polycyclic aromatic hydrocarbons in soil, and the degradation effect on polycyclic aromatic hydrocarbons is obvious, indicating that the bacterial strain is resistant t...

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Abstract

The invention belongs to the technical field of environmental engineering and microbiology engineering, and particularly relates to a strain for grading PAHs and an application thereof in combined contaminated soil remediation. The strain is an enterobacter cloacae tzyx2 with the collection number of CCTCC NO: M 2012240 in the China Center for Type Culture Collection (CCTCC), and is provided with the capacity to degrade the PAHs. The strain is applied to treatment of the PAHs and heavy metal polluted soil.

Description

technical field [0001] The invention belongs to the technical fields of environmental engineering and microbial engineering. In particular, a PAH-degrading Enterobacter cloacae ( Enterobacter cloacae ) tzyx2 and its application in complex contaminated soil remediation. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of persistent organic pollutants (POPs). PAHs pollution sources mainly come from waste machine oil, lubricating oil of electrical products, and hydrocarbon-containing substances such as wood, organic polymer compounds, paper, etc. from the dismantling of waste metal through incomplete combustion or thermal decomposition in a reducing atmosphere. And generated. Because PAHs and their derivatives have carcinogenicity, mutagenicity and teratogenicity, as well as acute toxicity and sublethal effects, they have attracted great attention. [0003] The remediation of polycyclic aromatic hydrocarbons (PAHs) contaminated soil is a res...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 于红艳张昕欣奚立民
Owner 深圳研源环境控股有限公司
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