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Ureibacillus sp. and bacterial agents and application thereof

A technology of bacillus and microbial inoculum, applied in the field of microorganisms, can solve the problems of high mining cost, high freezing point, difficult flow, etc., and achieve the effects of improving oil recovery rate, increasing fluidity, and increasing content

Active Publication Date: 2013-07-31
北京世纪金道石油技术开发有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Heavy oil, viscous oil, and high pouring oil are rich in waxes, colloids and asphaltenes, and have the characteristics of high freezing point and difficult flow, so they are difficult to mine and have high mining costs

Method used

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  • Ureibacillus sp. and bacterial agents and application thereof
  • Ureibacillus sp. and bacterial agents and application thereof
  • Ureibacillus sp. and bacterial agents and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1 Screening of Ureibacillus sp. JD-50 (CGMCC NO.5818) strain.

[0036] Take a mixed sample of oil and water from an oil well in a certain block of Liaohe Oilfield, oscillate and mix evenly, take 10ml with a pipette gun, and inoculate aseptically into 100ml of inorganic salt degradation basal medium (medium composition: NaCl 0.5%, (NH 4 ) 2 SO 4 0.1%, MgSO 4 ·7H 2 O 0.05%, NaNO 3 0.2%, KH 2 PO 4 0.5%, K 2 HPO 4 1.0%, FeSO 4 ·7H 2 O0.01%, yeast extract 0.1%, the balance is water, the pH value is 7.0-7.2; 121 ° C sterilization for 30 min), add 5 g (5%) crude oil, 60 ° C shaker speed 180 rpm, reciprocating shaking culture for 7 days. Take 10ml of the culture solution after 7 days, insert it into 100ml of new inorganic salt basic medium (crude oil 6%, the content of other components remains unchanged) at 60°C with a shaker at a speed of 180rpm, and culture with reciprocating shaking for 7 days. According to this procedure, five rounds of acclimatization...

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Abstract

The invention discloses a ureibacillus sp. and bacterial agents and an application thereof. According to the invention, a strain of ureibacillus sp. JD-50 is separated from a high-heat oil well water sample, and has a microbe preservation number of CGMCC NO.5818. The invention also discloses microbe bacterial agents formed by the ureibacillus sp. strains and a nutrient medium. The invention further discloses an application of the bacterial agents in petroleum exploitation. Test results show that the ureibacillus sp. or the bacterial agents of the invention can degrade and emulsify petroleum crude oil or diesel oil, effectively reduce the viscosity or freezing point of petroleum crude oil, and increase the fluidity of petroleum crude oil. As microbe oil-displacing agents, the ureibacillus sp. or the bacterial agents of the invention can significantly improve the petroleum recovery efficiency.

Description

technical field [0001] The present invention relates to a strain of microorganisms used in petroleum exploitation, in particular to a thermophilic urea bacillus capable of degrading and emulsifying petroleum crude oil and increasing the oil recovery rate and its microbial agent. The present invention further relates to their application in petroleum exploitation. It belongs to the field of microbial oil displacement agent. Background technique [0002] Petroleum is the blood of industry, the nerve artery of modern civilization, and plays an extremely important role in the development of human society. At the same time, oil is a non-renewable resource. With the increasing shortage of oil resources and the continuous increase of exploration costs, the efficiency of secondary water injection oil recovery technology has become low, which can no longer meet people's increasing demand for oil resources. In order to effectively utilize oil resources, countries all over the world a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K8/582C12R1/01
Inventor 王小通姜利滨李东安
Owner 北京世纪金道石油技术开发有限公司
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