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A technology for refining vegetable oil, weight, applied in the field of plant-based food and feed products, which can solve the problems of expensive use of clay, high disposal cost, danger, etc.

Active Publication Date: 2012-12-26
DUPONT NUTRITION BIOSCIENCES APS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Also the use of clay is expensive, not least because of the difficult, dangerous (prone to spontaneous combustion) disposal of used clay (i.e. waste) and thus high disposal costs

Method used

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example 1

[0249] Chlorophyllase from common wheat (Triticum triticum) in Bacillus subtilis Cloning and expression in

[0250] The nucleotide sequence (SEQ ID No.3) encoding wheat chlorophyllase (chlorophyllase) (SEQ.ID No.2, hereinafter referred to as wheat chlorophyllase (chlase)) is expressed in Bacillus subtilis, and it carries subtilis Bacillus alkaline protease (aprE) signal peptide (see Figure 17 ). For optimal expression in Bacillus, codon-optimized genes were ordered from GenScript Corporation, Piscataway, NJ 08854 (GenScript Corporation, Piscataway, NJ 08854, USA) construct (TRI_CHL).

[0251] Construct TRI_CHL consists of 20 nucleotides with a BssHII restriction site upstream of the wheat chlorophyllase coding region to allow fusion to the aprE signal sequence and a PacI restriction site downstream of the coding region for cloning into a Bacillus expression vector pBNppt.

[0252] Construct TRI_CHL was digested with BssHII and PacI and ligated to BssHII and PacI diges...

example 2

[0256] Cloning and Expression of Chlorophyllase from Chlamydomonas reinhardtii (Green Algae) in Bacillus subtilis

[0257] The nucleotide sequence (SEQ ID No.5) encoding Chlamydomonas chlorophyllase (chloryphyllase) (SEQ.ID No.4, hereinafter referred to as Chlamychlase (chlamy chlase)) was expressed in Bacillus subtilis, and Contains the Bacillus subtilis alkaline protease (aprE) signal peptide (see Figure 19 and 20 ). For optimal expression in Bacillus, codon-optimized gene constructs were ordered from GenScript Corporation, Piscataway, NJ 08854 (GenScript Corporation, Piscataway, NJ08854, USA) body (CHL_CHL).

[0258] Construct CHL_CHL consists of 20 nucleotides with a BssHII restriction site upstream of the Chlamydomonas chlorophyllase coding region to allow fusion to the aprE signal sequence and a PacI restriction site downstream of the coding region for cloning into Bacillus for expression Vector pBNppt.

[0259] Construct CHL_CHL was digested with BssHII and PacI...

example 3

[0263] Effect of Surfactant on Chlorophyllase Activity in Vegetable Oil

[0264] The activity of Arabidopsis chlorophyllase having the sequence of SEQ ID NO: 1 was tested in refined rapeseed oil by adding different surfactants including soybean lecithin. Prepare samples 1 to 6 containing the components defined in Table 1:

[0265] Table 1

[0266]

1

2

3

4

5

6

Refined Rapeseed Oil

g

10

10

10

10

10

10

Soy Lecithin

g

0.2

0.2

Sorbitan Monooleate

g

0.2

0.2

Sorbitan Trioleate

g

0.2

0.2

0.5mg / ml chlorophyll in acetone

µl

250

250

250

250

250

250

Chlorophyllase* in buffer*

ml

0

0.25

0

0.25

0

0.25

buffer*

ml

0.3

0.05

0.3

0.05

0.3...

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Abstract

In one aspect, provided herein is a process for refining a plant oil, comprising a step of contacting the oil with an enzyme which is capable of hydrolysing chlorophyll or a chlorophyll derivative, wherein the enzyme is contacted with the oil in the presence of at least 0.1% by weight phospholipid.

Description

technical field [0001] The present invention relates to the industrial processing of vegetable food and feed products, especially vegetable oils. The present invention can be used to reduce or eliminate pollution from chlorophyll and chlorophyll derivatives. Background technique [0002] Chlorophyll is a green pigment widely distributed throughout the plant kingdom. Chlorophyll is necessary for photosynthesis and is one of the most abundant organometallic compounds found on Earth. Thus many products from plants (including food and feed) contain considerable amounts of chlorophyll. [0003] For example, vegetable and peanut oils from oilseeds (eg soybean, palm seed or rapeseed (canola), cottonseed) usually contain some chlorophyll. However, the presence of high levels of chlorophyll pigments in vegetable oils is generally undesirable. This is because chlorophyll gives the oil an unsightly green color and can cause oxidation of the oil during storage, leading to deteriorat...

Claims

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Application Information

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IPC IPC(8): C11B3/00A23D9/04C11B3/04C11B3/06C11B3/14C12N9/18
CPCC11B3/003C11B3/14C11B3/04C11B3/001C12N9/18A23D9/04C12Y301/01014C11B3/06
Inventor R.米科森T.乔根森J.B.索伊
Owner DUPONT NUTRITION BIOSCIENCES APS
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