Mineral oil film-based bio-aerosol sampling method

Abstract

The invention discloses a mineral oil film-based bio-aerosol sampling method, which includes the following steps: (1) a sterilized culture dish is adopted, and hot sterilized agar medium is poured into the base of the culture dish in a sterile operating platform; (2) after the medium is coagulated, about 100Mu l of sterile mineral oil is added, and a sterilized glass rod is used for uniformly applying the mineral oil onto the upper surface of the agar medium; (3) an improved agar culture plate is directly put into a bio-aerosol sampler for sampling. The sampling method provided by the invention overcomes the defects of the conventional impact type sampling method, such as impact injuries of bio-particles, drying effect and organism insertion, and greatly increases the efficiency of bio-aerosol collection, and the method is simple and low in cost, and has a broad application prospect in the aspect of environmental bio-aerosol sampling.

Description

technical field [0001] The invention relates to a simple, high-efficiency and low-cost biological aerosol sampling method based on mineral oil film, which is an improvement to the international gold standard method (Andersen impact sampling method). Although the international gold standard method has been used for more than 50 years, its defects such as impact damage, drying effect, biological embedding, and rebound have not been improved. The present invention can make up for these defects of the international gold standard method and improve the collection of the gold standard method Efficiency, can be widely used in the sampling of environmental bioaerosols, etc. Background technique [0002] The agar medium-Anderson impact sampling method is an international standard bioaerosol collection method with a history of more than 50 years. As a standard method, it is widely used in the sampling and cultivation of bioaerosols. However, this method has the disadvantages of high ...

AI Technical Summary

Problems solved by technology

Fragile bioaerosols in ambient air may lose their culturability due to higher impact velocities, resulting in a decrease in the concentration of cultivable bacteria, leading to an underestimation of the ambient concentration level of culturable bioaerosols; high-flow air sampling will make agar cultures The surface of the substrate loses water, and then the physical microenvironment on the surface of the agar medium changes, which leads to a decrease in the cultivability of the collected bioaerosol

The above shortcomings will have a significant impact on the qualitative and quantitative analysis of bioaerosols, as well as the results of subsequent environmental exposure analysis.

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