Quantitative fluorescent multiplex PCR test kit for Epstein-Barr virus, and application thereof in nasopharyngeal carcinoma screening
A technology of multiple fluorescent quantification and detection kits, applied in the direction of microbial measurement/testing, fluorescence/phosphorescence, biochemical equipment and methods, etc., to achieve accurate prediction of disease risk, broad market, and high sensitivity
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[0035] 1. Oncogenes and mutation types detected:
[0036] The occurrence of NPC caused by EBV is related to some latent antigens of EBV, such as latent membrane protein (LMP) and EBV associated nuclear antigen-1 (EBNA-1). A large number of studies have focused on the analysis of these virus gene mutations in NPC patients. For example, the Affiliated Cancer Hospital of Sun Yat-Sen University surveyed 312,680 people in 17 towns in Sihui City, and found 60 NPC patients; and conducted the full sequence of EBV in South China for the first time at home and abroad. Assay work found that EBNA gene variants are associated with nasopharyngeal carcinoma. The project team found that only valine (V-val) EBNA1 (detection rate 98.1%) exists in nasopharyngeal carcinoma tissues, while EBNA1 V-val can be detected in peripheral blood lymphocytes and saliva. Both alanine (P-ala) and threonine (P-thr) EBNA1 were detected. And found that in peripheral blood lymphocytes and saliva, almost all V-va...
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