Single nucleotide polymorphism of microRNA-431 genes associated with lactation yield of dairy goats and detection and application thereof

A single nucleotide polymorphism, dairy goat technology, applied in DNA/RNA fragmentation, recombinant DNA technology, microbial determination/inspection, etc., can solve the problem of microRNA-431 that has not been reported, and achieve low cost and accuracy. High and low cost effect

Inactive Publication Date: 2013-10-02
NORTHWEST A & F UNIV
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Problems solved by technology

Wang et al. (Wang and Li., 2007) have analyzed that microRNA-431 has a down-regulation effect during pregnancy and lactation in mice by using microRNA chips, but the research on microRNA-431 in dairy goats has not been reported yet

Method used

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  • Single nucleotide polymorphism of microRNA-431 genes associated with lactation yield of dairy goats and detection and application thereof
  • Single nucleotide polymorphism of microRNA-431 genes associated with lactation yield of dairy goats and detection and application thereof
  • Single nucleotide polymorphism of microRNA-431 genes associated with lactation yield of dairy goats and detection and application thereof

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Embodiment Construction

[0035] In the present invention, firstly, primers are designed according to the conserved sequence of the microRNA-431 gene, and the genomic DNA pools of four goat varieties are respectively used as templates to carry out PCR amplification, and the PCR products are purified and sequenced. Then, analyze the sequence diagram and compare the sequences to screen out SNP sites; secondly, perform MluI-RFLP detection of polymorphic sites in the tested population; finally, perform population genetics according to the genotypes detected in the population Statistical analysis and association analysis of lactation volume screened out molecular markers closely related to lactation traits of dairy goats. The present invention will be described in detail below, which is an explanation of the present invention rather than a limitation.

[0036] a. Cloning of goat microRNA-431 gene DNA sequence and detection of its polymorphism

[0037] 1. Collection and processing of goat blood samples

[...

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Abstract

The invention discloses a single nucleotide polymorphism of microRNA-431 genes associated with lactation yield of dairy goats and detection and application thereof. The single nucleotide polymorphism of the genes comprises the single nucleotide polymorphism of which the 217th bit is T or C in a dairy goat microRNA-431 gene sequence showed in SEQ ID No. 1. Association analysis on the RFLP(restricted fragment length polymorphisms)and the lactation yield proves that: MluI polymorphic sites are obviously associated with the milk yield, and the milk yield and the body length of the third birth of which the genotype is TT are obviously higher than those of individuals of which the genotypes are TC and CC. According to the method, molecular genetic markers which are closely associated with the lactation properties of the dairy goats are screened and detected in a DNA (deoxyribose nucleic acid) level, and the method is used for the auxiliary selection and molecular breeding of the dairy goats and has an extremely important effect of improving the milk yield of the dairy goats.

Description

technical field [0001] The invention belongs to the technical field of dairy goat breeding, and relates to the single nucleotide polymorphism of microRNA-431 gene related to dairy goat milk production and its detection and its application as an auxiliary selection marker in dairy goat breeding. Background technique [0002] Goat milk is a health care product. As one of the three major milk sources of human beings, goat milk has high nutritional value and has the functions of nourishing the heart and lungs, benefiting the large intestine, nourishing the kidney and essence, nourishing yin and nourishing the stomach, curing thirst, and diet therapy. Curative food. Goat milk is known as "the king of milk" in the international nutrition circle. It is suitable for infants, adolescents, pregnant women, adults and the elderly (Chen Wei, 2009). In recent years, with the improvement of people's living standards in our country, people's demand for dairy products is also increasing. W...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68A01K67/027
Inventor 陈宏郭文娇李转见蓝贤勇孙雨佳薛璟潘虹
Owner NORTHWEST A & F UNIV
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