Method for overcoming adventitious buds vitrification of sisal hemp
A vitrification and sisal technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long hybrid breeding time, abnormal plant shape, poor anti-fungal ability, etc., and achieve simple and easy operation steps, Reduction of production cost, remarkable effect
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Embodiment 1
[0018] In June 2010, 30 H.11648 hemp calluses were inoculated on MS+6-BA3.0mg / L﹢NAA0.2mg / L and SH+6-BA3.0mg / L﹢NAA 0.2mg / L medium respectively For clumps, the culture conditions are: 12 hours of light, 12 hours of darkness, light intensity of 2000Lx, temperature of 26°C±2°C, relative humidity of 60%. After 2 months, the statistical test results showed that the callus adventitious bud induction rates were 88.3% and 89.2%, and the adventitious bud vitrification rates were 94.8% and 88.5%, respectively.
Embodiment 2
[0020] In July 2010, the plastic bottle cap of the culture container was changed to a sealing film with good air permeability. 30 H.11648 hemp calli were inoculated on 0.2mg / L medium, and the statistical test results after 2 months showed that the callus adventitious bud induction rate was 74.4% and 83.3%, and the adventitious bud vitrification rate was 68.9% respectively. % and 62.8%
Embodiment 3
[0022] From July to September 2010, with SH as the basic medium, NAA (0.1 mg / L, 0.5 mg / L, 1.0 mg / L), IBA (0.1 mg / L, 0.5 mg / L, 1.0 mg / L) and 6-BA (1 mg / L, 3 mg / L, 5 mg / L) concentration and combination were optimized, and other culture conditions remained unchanged. After 2 months, the statistical test results show that the callus adventitious bud induction rate is between 34.3-100.0%, and the adventitious bud vitrification rate is between 52.8% and 100%, of which 6-BA3.0mg / L, NAA0.1mg / The vitrification ratio of L and IBA0.1mg / L combination was better than other hormone combinations, the callus adventitious bud induction rate was 100.0%, and the vitrification ratio was 52.8%.
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