Method for overcoming adventitious buds vitrification of sisal hemp

Abstract

The invention relates to a method for overcoming adventitious buds vitrification of sisal hemp. According to the method, callus is directly inoculated to SZ culture medium to induce the adventitious buds; and the components of the SZ culture medium comprise 2267mg / L of potassium nitrate, 400mg / L of potassium dihydrogen phosphate, 150mg / L of ammonium sulfate, 400mg / L of calcium chloride dihydrate, 400mg / L of magnesium sulfate heptahydrate, 10mg / L of manganese sulfate monohydrate, 5mg / L of zinc sulphate heptahydrate, 5mg / L of boric acid, 1mg / L of potassium iodide, 0.1mg / L of sodium molybdate dehydrate, 0.2mg / L of copper sulfate pentahydrate, 0.1mg / L of cobalt chloride hexahydrate, 27.8mg / L of ferric sulfate heptahydrate, 37.3mg / L of disodium ethylenediamine tetraacetate, 0.8mg / L of thiamine hydrochloride, 1.0mg / L of pyridoxine hydrochloride, 1.0mg / L of nicotinic acid, 200mg / L of inositol, 3mg / L of glycine, 30000mg / L of sugar, 8000mg / L of carrageenan with a pH value of 5.8, 3.0 mg / L of 6-benzylaminopurine, 0.1mg / L of 3-indolebutyric acid, and 0.1mg / L of alpha-naphthylacetate. The SZ culture medium is used for inducing the adventitious buds from H.11648 hemp callus. The differentiation rate of the callus is above 90%, and the vitrification rate of the adventitious buds induced from the callus is below 8%, namely, the method can obviously overcome the occurrence of the adventitious buds vitrification of the sisal hemp.

Description

technical field [0001] The invention relates to a method for overcoming the vitrification of adventitious buds of sisal hemp, in particular to a method for overcoming the vitrification of adventitious buds in the establishment process of H.11648 hemp regeneration system. Background technique [0002] Sisal is a characteristic fiber crop in my country's hot areas, mainly distributed in Guangdong, Guangxi, Fujian, Hainan and Yunnan. In recent decades, sisal breeders have continuously introduced some new germplasm resources from abroad, and have also successively developed Sisal hybrid breeding work has been carried out, and some excellent germplasms have been obtained, but the serious shortage of sisal germplasm resources still exists. At present, H.11648 is the only leading variety in my country, with a planting area of ​​over 98%. Due to the poor anti-fungal ability of this variety, the continuous breeding of diseases and insect pests has seriously affected the high and stab...

AI Technical Summary

Problems solved by technology

Due to the poor anti-fungal ability of this variety, the continuous breeding of diseases and insect pests has seriously affected the high and stable yield of sisal

In addition, sisal has a long life cycle and only blooms once in a lifetime. The hybrid breeding time is long and the efficiency is low. Therefore, it is particularly important to use biotechnology to carry out genetic improvement and germplasm innovation of sisal

[0003] Some explorations have been made on the tissue culture of sisal at home and abroad, and the Asian maguey (A. cantala Robx ), Gray Leaf Sisal (A. fourcroydes Lem), Common Sisal (A. sisalana Perrine), Blue Sisal (A. tequilana Weber cultivar azul), A. parrasana Berger, A. tequilana, A. crassispina, A. duranguensisA. vera-cruz Mill., A. atrovirens, A. arizonica and Agave sisalana Perrine ex Engelmann and other in vitro regeneration systems and obtained a complete For regenerated plants, the South Subtropical Crops Research Institute of the Chinese Academy of Tropical Agricultural Sciences has also carried out research on the regeneration system of H.11648 hemp, and obtained a certain number of regenerated plants, but there is a certain degree of vitrification phenomenon, and the highest vitrification ratio reaches 100%. , The vitrified plants have abnormal morphology and cannot grow normally, and the vitrification of tissue culture seedlings has become a bottleneck in the process of plant tissue culture

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