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Antagonistic bacteria S20 for controlling peanut bacterial wilt, organic microbiological fertilizer prepared thereby, and application

A peanut bacterial wilt, organic fertilizer technology, applied in the field of microbial organic fertilizers, to increase income, save expenses, and reduce pesticide residues

Active Publication Date: 2012-12-19
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since there are no effective chemical pesticides at present, the prevention and control of peanut bacterial wilt mainly relies on crop rotation and selection of disease-resistant varieties

Method used

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  • Antagonistic bacteria S20 for controlling peanut bacterial wilt, organic microbiological fertilizer prepared thereby, and application
  • Antagonistic bacteria S20 for controlling peanut bacterial wilt, organic microbiological fertilizer prepared thereby, and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Isolation and Identification of Bacterial Strains

[0028] Ralstonia solanacearum (DSM No.9544), the pathogen of peanut bacterial wilt, was used as an indicator bacterium to isolate antagonistic bacteria from the rhizosphere soil of healthy peanut plants in Yingtan City, Jiangxi Province, where peanut bacterial wilt disease occurred. bacteria. Then through the pot experiment, the highly effective antagonistic strain S20 was finally screened out ( figure 1 ), the genus and species were identified, belonging to Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), and the strains were stored in 30% glycerol at -70°C. The antagonistic bacteria S20 was sent to the General Microorganism Center of China Microbiological Culture Collection Management Committee for preservation. The preservation date was August 15, 2011, and the preservation number was CGMCC No.5139.

[0029] Antagonistic bacteria S20, belonging to Bacillus amyloliquefaciens (Bacillus amyloliquefa...

Embodiment 2

[0030] Embodiment 2 Bacteria production

[0031] 1) The antagonistic bacteria S20 (CGMCC No.5139) strain is inoculated into the PDA culture medium for liquid fermentation production. The conditions for the fermentation production are: the initial pH range is 7.0-7.2, the culture temperature is 30°C, 170 rpm, and the fermentation is 48h After forming spores, the obtained S20 antagonistic bacteria fermentation broth contains bacteria or spores ≥ 5×10 9 a / ml;

[0032] The preparation method of the PDA culture medium used is as follows, taking the preparation of 1L medium as an example: Peel 200g of potatoes, cut into small pieces, put them in water and boil, boil for 30min, add 20g of ordinary sucrose to the filtrate after filtration, and dilute to 1000ml, adjust the pH value to 7.2-7.4, and sterilize at 121°C for 20min.

[0033] 2) Preparation of organic fertilizer (OF): use decomposed pig manure compost and amino acid organic fertilizer (product of Jiangsu Xintiandi Amino Aci...

Embodiment 3

[0036] Embodiment 3 field test

[0037] The experiment was carried out in the continuous cropping peanut field of Yingtan City, Jiangxi Province, and the following three treatments were set up, each with a distance of 150m 2 , repeated three times.

[0038] 1) CK (conventional fertilization)

[0039] 2) Use 75kg / mu microbial organic fertilizer

[0040] 3) Use 150kg / mu microbial organic fertilizer

[0041] Peanuts were planted at the beginning of May, and fertilization was carried out according to local routines (urea 20kg / mu, calcium magnesium phosphate fertilizer 40kg / mu, potassium chloride 20kg / mu), and microbial organic fertilizer was used as a base fertilizer at one time, and the insufficient N, P, K were supplemented with chemical fertilizers. Qi, to ensure the same nutrients for treatment and control. Harvest in mid-August. Investigate the occurrence of bacterial wilt at the maturity stage of peanuts, and calculate the control effect. The results showed that the biol...

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Abstract

The invention relates to antagonistic bacteria S20 for controlling peanut bacterial wilt, an organic microbiological fertilizer prepared by the antagonistic bacteria S20, and applications of the antagonistic bacteria S20, belonging to the intensive agriculture production technology. The antagonistic bacteria which obvious antagonism effect on pathogenic bacteria of the peanut bacterial wilt, namely bacillus amyloliquefaciens S20, is obtained by separation, and an organic microbiological organic fertilizer is prepared by the antagonistic bacteria S20 and an organic compost, and includes more than 2.0*109 parts / g S20 cells, 30.5g kg-1 of nitrogen content, 12.1g kg-1 of phosphorus content, and 20.4g kg-1 of potassium content. As an experiment shown, the antagonistic bacteria can quickly breed after feeding the organic microbiological fertilizer into the soil, and dominant species can be formed in the soil, and more than 60% of treatment rate of peanut bacterial wilt can be achieved.

Description

technical field [0001] The invention belongs to the field of agricultural microbes, and relates to an antagonistic bacteria S20 for preventing and treating peanut bacterial wilt, as well as microbial organic fertilizers prepared therefrom and applications thereof. Background technique [0002] Peanut bacterial wilt is a soil-borne disease caused by Ralstonia solanacearum. Once it occurs, it usually causes the death of 20%-30% of peanuts, and when it is severe, it can reach more than 60%, or even fail. Since there is no effective chemical pesticide at present, the prevention and control of peanut bacterial wilt mainly depends on crop rotation and selection of disease-resistant varieties. With the development of green agriculture, the focus of prevention and control of peanut bacterial wilt has gradually shifted to comprehensive control based on biological control. Screening of highly effective antagonistic strains is the core of biological control. At the same time, combining...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C05F17/00C12R1/07
CPCY02W30/40
Inventor 沈其荣沈标杨兴明冉伟
Owner NANJING AGRICULTURAL UNIVERSITY
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