Method for synchronously extracting corn germ oil and separation protein by using alcohol-assisted enzyme method

Abstract

The invention relates to a method for synchronously extracting corn germ oil and separation protein by using an alcohol-assisted enzyme method, and belongs to the technical field of cereal and oil bio-processing. The method comprises the following steps: (1) adopting an extrusion-expansion machine to carry out an extrusion-expansion pretreatment on corn germ to obtain an extrusion-expansion product; (2) carrying out enzymolysis on the extruded-expanded corn germ powder with a neutral protease, adjusting the pH value to 8.9 after completing the enzymolysis, carrying out centrifugation separation, and removing residues to obtain a mixing liquid of free oil, an emulsion and a hydrolysate; (3) adding an acid to the residue-removed mixing liquid, adjusting the pH value to 4.8, and carrying out centrifugation, wherein the resulting precipitate is high purity separation protein; and (4) carrying out an ultrasonic treatment on the separation protein-removed mixture, then adding ethanol, carrying out centrifugation, and carrying out eduction collection on oil to obtain the corn germ oil. According to the method of the present invention, high quality green corn germ oil and high purity separation protein can be synchronously obtained, the required equipment is simple, operation is safe, the resulting corn germ oil does not have solvent residue, high quality and high nutritional value oil and high purity degeneration separation protein are obtained, the separation protein extraction rate can be 97.64%, and the total oil extraction rate can be 99.54%.

Description

technical field [0001] The invention belongs to the bioprocessing technology of grain and oil, and mainly relates to a method for synchronously extracting corn germ oil and protein isolate by an alcohol-assisted enzymatic method. Background technique [0002] my country is a large corn producing country. In 2008, 2009, 2010 and 2011, the annual corn production in my country was 165 million tons, 163 million tons, 168 million tons and 182.5 million tons respectively, and a large amount of corn germ was produced every year. Corn germ oil (corn germ oil) is a kind of grain germ oil extracted from corn germ. It is rich in nutrition, unique in flavor, easy to digest (the absorption rate of human body can reach more than 97%), and the content of unsaturated fatty acid is more than 90%. , which contains about 40% oleic acid, about 50% linoleic acid, linolenic acid (2.9%-3.2%), and corn germ oil is also rich in vitamins (VE, VA), of which VE content ranks first in vegetable oil, abo...

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Problems solved by technology

[0007] So far, there is no process that can simultaneously extract corn germ oil and protein isolate, and it is the first time to extract protein first and then extract oil, which is different from the traditional process of extracting oil first and then extracting protein, and Combining extrusion expansion with water enzymatic method, and combining ultrasonic wave and ethanol treatment for demulsification, there is almost no research on this technology in the corn germ oil extraction process

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