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Method for extracting natural algicide from Acorus calamus L and algae inhibition method

The technology of an algae inhibitor and water calamus, which is applied in the field of algae inhibitory active ingredients, can solve the problems of difficulty in separation and high cost, and achieve the effects of strong environmental affinity, low cost, and simple and easy extraction method.

Inactive Publication Date: 2014-09-24
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the disadvantages of difficult separation and high cost in the actual use of existing plant algae inhibitors, the present invention provides a method for extracting algae inhibitory active ingredients from calamus calamus and an algae inhibitory method, which have a wide range of sources and good algae inhibitory effect ,low cost

Method used

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  • Method for extracting natural algicide from Acorus calamus L and algae inhibition method
  • Method for extracting natural algicide from Acorus calamus L and algae inhibition method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: The tuberous rhizomes of Acorus calamus were collected, dried and crushed. Weigh 50g of the crushed calamus rhizome, extract 2-3 times at 50C, 12 hours each time. Filter, combine the petroleum ether extracts, and concentrate by rotary evaporation at 35°C to obtain the algae inhibitory active component A. The active component A was subjected to acute toxicity experiments on sterile cultured single-celled Microcystis aeruginosa, colony Microcystis aeruginosa, A. algae and Anabaena algae, set several concentration gradients, set blanks and three The groups are in parallel and observed once every 24 hours for three consecutive days. The results are expressed as the inhibition rate of 96 hours. The results are as follows:

[0031] The anti-algae active ingredient A, when the mass volume concentration reaches 50mg / L (then 1 liter of water contains 50mgA), its inhibition rate of single-celled Microcystis aeruginosa reaches 90%; when the concentration reaches 100mg / L, ...

Embodiment 2

[0032] Example 2: The tuberous rhizomes of Acorus calamus were collected, dried and crushed. Weigh 50g of crushed calamus rhizome, extract 2-3 times at 50 degrees, 12 hours each time. After filtration, the ethyl acetate extracts were combined, and concentrated by rotary evaporation at 35° C. to obtain the algal inhibitory active component B.

[0033] The active ingredient B was subjected to acute toxicity experiments on sterilely cultured single-celled Microcystis aeruginosa, colony Microcystis aeruginosa, A. algae and Anabaena algae, set several concentration gradients, set blanks and three The groups are in parallel and observed once every 24 hours for three consecutive days. The results are expressed as the inhibition rate of 96 hours. The results are as follows:

[0034] Anti-algae active ingredient B, when the concentration reaches 50mg / L, its inhibition rate on single-celled Microcystis aeruginosa reaches 91%; when the concentration reaches 100mg / L, its inhibition rate on po...

Embodiment 3

[0035] Example 3: The tuberous rhizomes of Acorus calamus were collected, dried and crushed. Weigh 50g of crushed calamus rhizome, extract 2-3 times at 50 degrees, 12 hours each time. Filter, combine the absolute ethanol extracts, and concentrate by rotary evaporation at 35°C to obtain the algal inhibitory active component C.

[0036] The active ingredient C was subjected to acute toxicity experiments on sterilely cultured single-celled Microcystis aeruginosa, colony Microcystis aeruginosa, A. algae and Anabaena algae, set several concentration gradients, set blanks and three The groups are in parallel and observed once every 24 hours for three consecutive days. The results are expressed as the inhibition rate of 96 hours. The results are as follows:

[0037] Anti-algae active ingredient C, when the concentration reaches 50mg / L, its inhibition rate on single cell Microcystis aeruginosa reaches 95%; when the concentration reaches 100mg / L, its inhibition rate on population Microcyst...

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Abstract

The invention discloses a method for extracting active components for algae inhibition from Acorus calamus L tubers. According to the invention, an organic solvent is directly used to extract the active components so as to obtain the algicide, or the organic solvent is used for sequential extraction to obtain the algicide. The direct extraction method of the organic solvent comprises the following concrete steps of: extracting washed, dried and crushed Acorus calamus L tubers for 2-5 times respectively by using petroleum ether, ethyl acetate and anhydrous ethanol, filtering, and condensing to obtain the active components A, B and C for algae inhibition. The sequential extraction method of the organic solvent comprises the following concrete steps of: extracting by the use of anhydrous ethanol to obtain an extract C, dissolving the extract C in 80% of a methanol aqueous solution, carrying out isopyknic extraction for 2-3 times by the use of petroleum ether, condensing the petroleum ether layer to obtain a first active component for algae inhibition, diluting residual methanol aqueous solution to 50% by the addition of water, carrying out isopyknic extraction for 2-3 times by the use of ethyl acetate, and condensing the ethyl acetate layer to obtain a second active component for algae inhibition. The extraction method is simple and easy to operate. The extracted active components, which are used as the algicide, are cheap and nontoxic, require low cost, and are suitable for practical popularization and application. The algicide can be used without the restrictions of region, solar term, temperature and the like.

Description

Technical field [0001] The invention belongs to the field of water pollution control, and specifically designs a method for extracting active ingredients from natural plant calamus tuber for inhibiting algae. Background technique [0002] There are two main ideas for water bloom control technology: controlling nutrient elements such as nitrogen and phosphorus and controlling algae. The current algae control technology includes physical algae control technology, chemical algae control technology and biological algae control technology, of which the most commonly used method is chemical method. The existing chemical algaecides are mainly copper sulfate. The mechanism is to use the toxicity of copper ions to interfere with the photosynthetic reaction system of algae, making it unable to perform photosynthesis normally, and ultimately achieve the purpose of killing algae. However, copper ions are heavy metals Ions, which are not selective, are absorbed or ingested by other aquatic p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/40A01P13/00A01P1/00
Inventor 张胜花赵静孙珮石
Owner YUNNAN UNIV
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