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Chicken semen diluent as well as preparation method and application thereof

A technique for diluting liquid and semen, applied in the fields of application, preservation of human or animal body, animal husbandry, etc., can solve the problems of high reagent price and unsatisfactory effect, etc., to improve sperm motility, reduce sperm deformity rate, and prolong survival time. Effect

Inactive Publication Date: 2012-12-12
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although many domestic units and scholars have conducted relevant research on chicken semen and its dilutions, and have made some progress, currently there are mainly Beltsville poultry semen dilution (BPSE), Lake's preservation solution formula and Brown's solution for chicken semen at room temperature. , there is only Beijing Poultry Diluent (BJJX) in China, but due to the differences in the species of chickens studied, the breeding environment, etc., the semen characteristics of chickens are also different. Reagent prices are higher

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A dilution of chicken semen, using sterilized distilled water as a solvent, containing fructose, citric acid, sodium glutamate, potassium chloride, dipotassium hydrogen phosphate, Tris, bovine serum albumin, vitamin B 12 , streptomycin and penicillin, the concentrations of the above components are: fructose 10 g / L, citric acid 0.4 g / L, sodium glutamate 4 g / L, potassium chloride 1 g / L, dipotassium hydrogen phosphate 8 g / L , Tris4 g / L, bovine serum albumin 3 g / L, vitamin B 12 0.8 g / L, penicillin 100IU / mL, streptomycin 0.1 mg / mL; the chicken semen dilution has a pH value between 6.8-7.2 and an osmotic pressure of 300-400mOs / kg, see Table 1.

[0014] Table 1 Formula of Chicken Semen Diluent

[0015] Element concentration fructose 10g / L citric acid 0.4 g / L sodium glutamate 4g / L potassium chloride 1g / L Dipotassium phosphate 9g / L Tris 0.6 g / L bovine serum albumin 3g / L Vitamin B 12 0.8g / L penicillin 10...

Embodiment 2

[0019] Weigh 10 g of fructose, 0.4 g of citric acid, 4 g of sodium glutamate, 1 g of potassium chloride, 8 g of dipotassium hydrogen phosphate, 4 g of Tris, 3 g of bovine serum albumin, and 0.8 g of vitamin B 12 Dissolve in a small amount of sterilized distilled water, transfer it to a 1000ml volumetric flask after dissolving, and add penicillin and streptomycin to a final concentration of penicillin of 100IU / mL and a final concentration of streptomycin of 0.1mg / mL. All preparation processes are carried out under sterile conditions. Store at 4°C until use. When in use, preheat the diluent to 37°C and mix it with chicken semen at a ratio of 1:1 to 2.

Embodiment 3

[0021] The roosters with strong physique, strong libido, and sperm collection training are selected for semen collection. According to the test operation of the diluent, the chicken semen diluent and the semen stock solution of the present invention are used to carry out a comparative test. Collect well-trained breeding roosters for semen collection, and after obtaining mixed semen, dilute with chicken semen diluent of the present invention, and the dilution ratio is diluent:sperm=1:1. Stored at 25°C, the hen is artificially inseminated, and the insemination operation is performed by turning the anus, and the insemination volume is 20-25 μL. The insemination process is completed within 1 hour. Repeat the test 3 times. The results show that: after diluting and inseminating the chicken semen with the chicken semen diluent of the present invention, the fertilization rate of the hen is 91.71%, while the fertilization rate of the stock solution is 92.47%, and there is no signific...

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Abstract

The invention discloses Chicken semen diluent as well as a preparation method and application thereof, and belongs to the technical field of animal breeding. Chicken semen is diluted for normal temperature preservation and artificial insemination. The Chicken semen diluent takes sterile distilled water as a solvent and contains fructose, citric acid, sodium glutamate, potassium chloride, dipotassium hydrogen phosphate, Tris, bovine serum albumin, VB12 (Vitamin B12), streptomycin and penicillin. The preparation method comprises the following steps of: dissolving the fructose, citric acid, sodium glutamate, potassium chloride, dipotassium hydrogen phosphate, Tris, bovine serum albumin and VB12 into a small amount of sterile distilled water under the sterile condition, adding the penicillin and the streptomycin, adding the sterile distilled water, mixing uniformly to obtain the Chicken semen diluent, and preserving at the temperature of 4 DEG C; and preheating the Chicken semen diluent to the temperature of 37 DEG C, and mixing the Chicken semen diluent and Chicken semen, wherein the mass ratio of the Chicken semen to the Chicken semen diluent is (1:1)-(1:2). The Chicken semen diluent has the advantages of simplicity and convenience in preparation, convenience in material acquisition, low cost and easiness in popularization.

Description

technical field [0001] The invention relates to a diluent of chicken semen, which belongs to the technical field of animal reproduction and is used for normal temperature preservation and artificial insemination of dilute chicken semen. Background technique [0002] With the rapid development of the modern poultry industry, the scale and intensification of poultry farming are getting higher and higher, and the artificial insemination of poultry is becoming more and more important. In my country, the three-dimensional cage technology of chickens and artificial insemination technology are mutually causal, providing a broad space for the development of poultry farming with chickens as the main body. For the hens that produce breeding eggs, generally one time of insemination is required every 5 days, and 73 times of insemination are required in one year. In 2011, about 9 billion broiler chickens were slaughtered in my country, and the average stock of ancestral egg breeders exce...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 赵振华黎泰丰薛龙岗黄华云李春苗武子寅温彦涛
Owner JIANGSU INST OF POULTRY SCI
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