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Food intolerant serological specificity IgG detection kit and preparation method thereof

A kit and specific technology, which is applied in the field of food intolerance serum-specific IgG detection kit and its preparation, can solve the problems of inability to meet the individual needs of patients, and achieve improved versatility and utilization, convenient operation, Effects of avoiding detection and reagent waste

Inactive Publication Date: 2012-12-05
天津中企华科生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the deficiency that the food allergy enzyme-labeled reaction plate prepared by the traditional direct coating mode cannot meet the individual needs of patients, the present invention introduces a biotin-streptavidin indirect coating mode, and the coating production process of the enzyme-labeled reaction plate make improvements

Method used

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  • Food intolerant serological specificity IgG detection kit and preparation method thereof
  • Food intolerant serological specificity IgG detection kit and preparation method thereof
  • Food intolerant serological specificity IgG detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] A food intolerance serum-specific IgG enzyme-linked immunoassay kit, comprising the following components:

[0056] (1) Enzyme-labeled reaction plate pre-coated with biotinylated bovine serum albumin-streptavidin

[0057] (2) A variety of biotin-labeled food allergens, including biotin-labeled milk allergens, egg allergens, shrimp allergens, wheat allergens, peanut allergens, cashew nut allergens, crab Allergens and soy allergens;

[0058] (3) Biotin-labeled anti-human IgG antibody (goat anti-human IgG antibody, ABCAM Company, Cat. No. ab48386)

[0059] (4) Human IgG standard substances of different concentrations (pure IgG (ABCAM company, product number ab91102) was prepared into 5 different concentrations with pH 7.40.1M PBS containing 1% calf serum as standard substances, and the concentrations of the standard substances were respectively "0U / ml", "50U / ml", "100U / ml", "200U / ml", "400U / ml", the standard products are calibrated by international quality control serum.)...

Embodiment 2

[0134] A method for using the kit prepared in Example 1, comprising the steps of:

[0135] 1. According to the purpose of the test and the patient's medical history, the tester can freely select one or more allergens to be tested from the provided biotin-labeled food allergens for item combination, such as milk allergens , egg allergen, shrimp allergen, wheat allergen, peanut allergen, cashew nut allergen, crab allergen and soybean allergen or a combination of several.

[0136] 2. Use the kit as follows:

[0137] (1) Preparation Take out the enzyme-labeled reaction plate (pre-coated with biotinylated bovine serum albumin-streptavidin) as needed. The number of test wells is: number of samples × coefficient + 5 standard curve points + positive quality control serum, and the coefficient is the number of food allergens tested for each sample. Place the biotin-labeled food allergen reagent, enzyme-labeled anti-human IgG antibody, chromogenic solution A, chromogenic solution B, st...

Embodiment 3

[0153] The performance detection of kit of the present invention is as follows:

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Abstract

The invention provides a food intolerant serological specificity IgG detection kit and a preparation method thereof. The kit comprises an ELISA (enzyme linked immunosorbent assay) plate precoated with biotinylation bovine serum albumin and streptavidin (1), a biotin-labelled food allergen (2), a biotin-labelled anti-human IgG antibody (3), a human IgG standard product (4), a positive quality control blood serum (5), an enzyme-labelled anti-human IgG antibody (6), a common enzyme-labelled cleaning fluid (7), a development solution A (8), a development solution B (9) and a stop solution (10). By utilizing the kit provided by the invention, detection personnel can flexibly select food allergen required to be detected according to specific conditions of a detected person and simultaneously add the biotin-labelled allergen and blood serum to be detected into a micropore plate, so that instant coating can be realized; and the coating and detection processes can be synchronously carried out, so that the detection cost can be lowered, the detection flexibility can be improved, and the individual detection is realized.

Description

technical field [0001] The invention belongs to the field of enzyme-linked immunoassay detection, and in particular relates to a food intolerance serum-specific IgG detection kit and a preparation method thereof. Background technique [0002] Allergic disease is a common disease in modern society. It is caused by widely existing allergens. About 5-10% of people in our country are infested by allergens. Substances that trigger an allergic reaction are called allergens, and food is an important allergen. Food allergies can be caused by the human body’s sensitivity to food. At present, there are two main types: the immediate allergic reaction mediated by IgE, which usually occurs quickly and the symptoms are obvious; the delayed allergic reaction mediated by IgG, which is usually Symptoms of reactions such as chronic fatigue, arthritis, hives, eczema, headaches, gastrointestinal disorders, and other chronic symptoms appear hours or days after exposure to food. Usually, food-i...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 李会强王高升相平
Owner 天津中企华科生物科技发展有限公司
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