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Application of single nucleotide polymorphism rs3888188 to detection of tuberculosis susceptibility

A rs3888188, polymorphism technology, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problem of no research proof and so on

Inactive Publication Date: 2012-12-05
BEIJING CHILDRENS HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently, no studies have demonstrated the association of interferon-induced transmembrane protein 3 (IFITM3) and its gene IFITM3 with tuberculosis susceptibility

Method used

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  • Application of single nucleotide polymorphism rs3888188 to detection of tuberculosis susceptibility
  • Application of single nucleotide polymorphism rs3888188 to detection of tuberculosis susceptibility
  • Application of single nucleotide polymorphism rs3888188 to detection of tuberculosis susceptibility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. The single nucleotide polymorphism of rs3888188 is associated with tuberculosis (case-control analysis)

[0032] PCR amplification was performed on the IFITM3 gene core promoter sequence (as shown in Sequence 3) of 951 normal controls (i.e., normal control group) and 368 cases of tuberculosis (i.e., tuberculosis case group) DNA samples from Han children in northern China, The amplified PCR products were separated by electrophoresis on 1.5% agarose gel, and the 346bp band was recovered and purified for sequencing.

[0033] Use the online Shesis software (http: / / analysis.bio-x.cn / SHEsisMain.htm) to perform statistics on the allele frequency and genotype frequency of the SNP sites found in the sequencing, and perform tuberculosis for the allele and genotype respectively. Chi-square test and calculation of odds ratio (odds ratio), inter-site linkage analysis, haploid analysis and other genetic statistical analysis of the case group and the normal control group ob...

Embodiment 2

[0052] Embodiment 2, IFITM3 gene is tuberculosis susceptibility gene

[0053] 1. Effect of SNP on IFITM3 gene promoter on IFITM3 gene expression

[0054] The three SNP sites rs3888188, rs6598045 and rs7478728 were constructed as GTT, TTC or TCT haplotype promoter sequences into the corresponding dual luciferase reporter gene detection vector pGL3-basic (Promega, E1751) for dual luciferase Enzyme reporter gene experiment, to compare the transcriptional activities of the above-mentioned different haplotype promoters, as follows:

[0055] 1. Design of primers

[0056] The upstream primer starts at 244 bp upstream of the IFITM3 gene transcription start site, and the downstream primer ends at 102 bp downstream of the IFITM3 gene transcription start site (the base before the translation start site ATG). NheI (GCTAGC) and XhoI (CTCGAG) were selected as the upstream and downstream restriction sites respectively, and the primer sequences are as follows:

[0057] Upstream primer: 5'-...

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Abstract

The invention discloses an application of single nucleotide polymorphism rs3888188 to detection of tuberculosis susceptibility. The SNP (Single Nucleotide Polymorphism) is the rs3888188 and is positioned in a human IFITM3 (interferon induced transmembrane protein) gene core promoter region (namely the upstream 103bp of a transcription initiation site / the upstream 204bp of a translation initiation site ATG), the rs3888188G is a risk factor of tuberculosis susceptibility, and the polymorphism of the rs3888188 is highly related with tuberculosis. When the genotype of the site is GG, the tuberculosis susceptibility or tuberculosis causing risk of an individual to be detected is increased. The application is of significant meaning and value in the aspects of diagnosing and treating the tuberculosis to reasonably prevent the tuberculosis.

Description

technical field [0001] The invention relates to the application of single nucleoside polymorphism rs3888188 in detecting tuberculosis susceptibility. Background technique [0002] Tuberculosis (TB) is one of the three major infectious diseases of global concern today, which seriously threatens the health of the public. The number of tuberculosis patients in my country ranks second in the world. Carrying out research on genetic susceptibility to tuberculosis has important scientific value and social benefits to eliminate tuberculosis, reduce mortality, protect people's health, and promote the healthy and sustainable development of the capital's economy and health services. [0003] The main pathogenic bacteria of tuberculosis is Mycobacterium tuberculosis (MTB), and the pathogenesis of tuberculosis is the result of a combination of multiple factors. The occurrence, development and clinical outcome of tuberculosis are not only controlled by the environment and MTB virulence, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 申阿东申晨吴喜蓉孙琳焦伟伟綦辉
Owner BEIJING CHILDRENS HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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