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A carbon source combination for improving microbial oil yield

A technology for microorganisms, microbial fermentation, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc.

Active Publication Date: 2016-02-03
WILMAR SHANGHAI BIOTECH RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the method of reducing erucic acid and producing LCPUFA at the same time.

Method used

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  • A carbon source combination for improving microbial oil yield
  • A carbon source combination for improving microbial oil yield
  • A carbon source combination for improving microbial oil yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] High erucic acid rapeseed oil is used as the carbon source of the fermentation medium, and it can be converted into erucic acid-free and rich in LCPUFA oil products through the fermentation of Schizochytrium spATCC20888.

[0074] The cultivation steps are as follows:

[0075] (1) Streak inoculation of the glycerol tube bacteria stored in the ultra-low temperature refrigerator to the slant for activation. The activation medium is: glucose 5g / L, peptone 1g / L, yeast powder 1g / L, agar powder 18g / L, sea salt 30g / L. Incubate at 28°C for 40 hours.

[0076] (2) Pick the activated single colony and inoculate it into the seed liquid culture medium (recipe is shown in Table 1), in a liquid volume of 50ml / 250ml Erlenmeyer flask, and culture on a shaker at 28°C and 200rpm for 48 hours. The concentration of the seed liquid is about 15-20g / L dry weight.

[0077] Table 1 Seed medium formula (g / L)

[0078] project

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Embodiment 2

[0088] Using oil or oil refining by-products as a carbon source for fermentation, through the fermentation of Schizochytrium sp., it was found that the palm oil deodorized distillate PFAD was very efficient as a carbon source, and the fermented oil had very good nutritional value.

[0089] The cultivation steps are the same as the parts (1), (2), (3) and (4) of the embodiment.

[0090] Oil carbon sources were selected respectively: palm oil (Malaysia Pasir Gudang Edible Oil Company), palm oil deodorized distillate PFAD (Malaysia Pasir Gudang Edible Oil Company), palm kernel oil (Malaysia Pasir Gudang Edible Oil Company), Kano Pull (canola) vegetable oil (Shanghai Kerry Food Co., Ltd.), soybean oil (Shanghai Kerry Food Co., Ltd.), corn oil (Shanghai Kerry Food Co., Ltd.).

[0091] Palm oil deodorization distillate PFAD contains about 47.2% by weight palmitic acid (16:0), about 4.5% by weight stearic acid (18:0), about 36.7% oleic acid (18:1), about 9.7% by weight % of linoleic...

Embodiment 3

[0099] Comparing various types of oil and glucose to form a composite carbon source, comparing the biomass density, lipid yield, and lipid DHA yield of Schizochytrium algae.

[0100] Cultivate steps with (1), (2), (3), (4) of embodiment 1

[0101] Table 5 Biomass, lipid yield, DHA content in lipid, and DHA yield obtained from different composite carbon sources

[0102]

[0103] From the data in Table 5, it can be seen that when high erucic acid rapeseed oil (Sichuan Kerry Food Co., Ltd.), anchovy oil (Tecnológicade Alimentos S.A. company in Peru), tuna oil (LYSICOMPANY in Iceland) and glucose co-cultured with fatty acid carbon number greater than 18 Compared with soybean oil, canola oil, corn oil, and palm oil (the source is the same as that in Example 2) whose fatty acid carbon number is equal to or less than 18, the algae oil produced by Schizochytrium significantly improves the DHA yield.

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Abstract

The present invention relates to a carbon source combination for increasing microbial oil yield. Specifically the invention relates to an application of a culture medium for lipid-producing microorganism fermentation, erucidic acid-containing oil and a palm oil deordorization distillate in the field of increase of microbial oil yield, and the like.

Description

technical field [0001] The invention belongs to the field of lipid production by microbial fermentation. Specifically, the present invention relates to a carbon source used for microbial fermentation to produce lipid (especially DHA oil), and a method for using the carbon source to produce lipid by microbial fermentation. Background technique [0002] Long chain polyunsaturated fatty acids (LCPUFA) such as EPA, DPA, DHA, ARA have a very important contribution to people's health. At present, the ways for people to ingest LCPUFA are still limited to deep-sea fish oil, microbial oil and so on. Because the source is limited, but people's demand is very huge, cause the price of LCPUFA product to be still very expensive at present. [0003] The most costly part of the culture medium is the carbon source in the production of microbial oil by fermentation. Common carbon sources are carbohydrates such as glucose and starch. [0004] In the process of oil processing, many oil by-p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12N1/20C12N1/14C12R1/01C12R1/63C12R1/645
Inventor 常桂芳田桂尾吴清杭戴小军
Owner WILMAR SHANGHAI BIOTECH RES & DEV CENT
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