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Efficient regeneration system method for mint internode stems

A high-efficiency regeneration and internode technology, applied in the directions of plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of short shoot tip propagation time, unsuitable for large-scale expansion of mint varieties, etc., and achieve short regeneration period, good repeatability

Inactive Publication Date: 2012-11-21
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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AI Technical Summary

Problems solved by technology

At present, the rapid propagation system for domestic mint varieties mainly uses shoot tips as regeneration materials, but this method is suitable for large-scale multiplication of mint varieties and is not suitable for the establishment of genetic transformation methods.

Method used

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  • Efficient regeneration system method for mint internode stems
  • Efficient regeneration system method for mint internode stems
  • Efficient regeneration system method for mint internode stems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The regeneration of adventitious buds in internode stem segments of mint variety '738' is carried out in several steps in the following order:

[0032] 1. Collection of explants and establishment of sterile system

[0033] Mint terminal buds were collected from March to June, soaked in washing powder solution for 15 minutes, and rinsed with running water for 30 minutes. Before inoculation, sterilize with 75% (V / V) alcohol for 30 seconds, soak in 0.2% (m / V) HgCl2 solution for 15 minutes, and rinse with sterile water three times. After absorbing the surface moisture, peel off the outer leaves, cut into 0.5cm long shoot tips, and inoculate them in MS medium containing 1.0mg L-1BA and 0.2mg L-1NAA. The medium contains 5.6g ·L-1 agar, 30g·L-1 sucrose, pH5.5~5.8, sterilized at 121°C for 20min. The culture temperature is 25°C, the light time is 8-10 hours, and the light intensity is 1500-2000lx. After three weeks of cultivation, healthy and sterile plants were selected and ...

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Abstract

An efficient regeneration system method for mint internode stems is established by using morphological second, third and fourth internode stems of an aseptic mint seedling as material, inoculating the material to adventitious bud induction medium, and researching by key technologies such as induction, elongation growth, subculture, seedling strengthening, rooting and the like. The efficient regeneration system method for mint internode stems has the advantages that regeneration rate of induced adventitious buds is up to 95%, stability is high, operation is simple, regeneration cycle is short, and the regeneration system can serve as a receptor system for genetic transformation of Agrobacterium Tumefaciens.

Description

technical field [0001] The invention relates to the establishment of a method for efficient regeneration of mint internode stem segments technical background [0002] The stems and leaves of peppermint (Mentha haplocalyx Briq.) are rich in volatile oil, and are widely used in food, medicine, cosmetics, spices, tobacco and other industries. The whole herb of mint is used as medicine to treat wind-heat cold, the beginning of wind and temperature, headache, red eyes, sore throat, sore throat, sore tongue, toothache, urticaria, rubella, etc. Mint is one of the important spices and medicinal plants in China. It is widely cultivated in Jiangsu, Anhui, Jiangxi and other places, and has become an important agricultural special economic crop in the local area. However, the phenomenon of single species and species degeneration in mint production has made mint breeding work more and more attractive. The use of transgenic technology to cultivate fine new varieties of mint has the adva...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李维林于盱梁呈元王海棠刘艳
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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