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MicroRNA (micro ribonucleic acid) for regulating gene expression of PTEN (phosphatase and tensin homolog)

A kind of gene expression, tiny technology, applied in the direction of DNA / RNA fragment, recombinant DNA technology, etc., can solve the problems such as the lack of miR-20b and PTEN gene correlation, different functions, and the difficulty of miRNA.

Inactive Publication Date: 2012-11-14
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]Although it is known in the art that certain microRNAs are related to tumors, there are many kinds of miRNAs known in the art with different functions, and we should select from them It is difficult to screen out specific miRNAs that are related to tumors and can be used as pathogenesis, treatment options and prognosis
At present, there is no report on the correlation between miR-20b and PTEN gene in this field

Method used

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  • MicroRNA (micro ribonucleic acid) for regulating gene expression of PTEN (phosphatase and tensin homolog)
  • MicroRNA (micro ribonucleic acid) for regulating gene expression of PTEN (phosphatase and tensin homolog)
  • MicroRNA (micro ribonucleic acid) for regulating gene expression of PTEN (phosphatase and tensin homolog)

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Embodiment Construction

[0024] The above solution will be further described below in conjunction with specific embodiments. It should be understood that these examples are used to illustrate the present invention and not to limit the scope of the present invention. The implementation conditions used in the examples can be further adjusted according to the conditions of specific manufacturers, and the implementation conditions not indicated are usually the conditions in routine experiments.

[0025] 1. Reagents and materials

[0026] 1. Reagents

[0027] Fetal bovine serum (Hyclone, USA); complete medium: add 100ml of fetal bovine serum, 0.15g of L-glutamine, 10.0ml of 2-mercaptoethanol (5×10 -3 mol / L); liposome 2000 (Invitrogen, USA); penicillin, streptomycin (Shanghai Sangon Biotechnology Co., Ltd); TRIzol (Invitrogen, USA); agarose (LP0028A, Oxoid Ltd, UK); gel Electrophoresis sample solution and ethidium bromide (EtBr) (Shanghai Sangon Biotechnology Co., Ltd.); gel recovery DNA kit an...

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Abstract

The invention discloses microRNA (micro ribonucleic acid) for regulating gene expression of PTEN (phosphatase and tensin homolog). The microRNA is nucleic acid, functional segment or variant including a sequence of 5'-caaagugcucauagugcagguag-3'. The microRNA is miR-20b. In-vitro biological function tests show that hsa-miR-20b is capable of inhibiting molecular expression of the PTEN by bonding with 3'-UTR (untranslated regions) of the PTEN gene, and antitumor effect can be achieved by regulating a signal path of the PTEN and / or expression and functions of the miR-20b.

Description

technical field [0001] The invention belongs to the field of biotechnology and medical technology, specifically, the invention relates to the technical field of gene expression regulation, in particular to the use of microRNA to regulate PTEN gene expression. Background technique [0002] The gene was discovered in 1997 as a tumor suppressor gene with dual-specificity phosphatase activity (Li J, Yen C, Liaw D, et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science 1997, 275(5308):1943-7), regulate cell growth and proliferation by negatively regulating PI3K / Akt pathway (Wu H, Goel V, Haluska FG. PTEN signaling pathways in melanoma. Oncogene 2003, 22 (20):3113-22). Studies have found that the PTEN gene is mutated or lost to varying degrees in a variety of tumors, such as colorectal cancer, prostate cancer, endometrial cancer, thyroid cancer and melanoma. Loss of PTEN molecular expression is significantly associa...

Claims

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Application Information

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IPC IPC(8): C12N15/113
Inventor 汪维鹏陈兰心朱健洁
Owner SUZHOU UNIV
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