MicroRNA (micro ribonucleic acid) for regulating gene expression of PTEN (phosphatase and tensin homolog)
A kind of gene expression, tiny technology, applied in the direction of DNA / RNA fragment, recombinant DNA technology, etc., can solve the problems such as the lack of miR-20b and PTEN gene correlation, different functions, and the difficulty of miRNA.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0024] The above solution will be further described below in conjunction with specific embodiments. It should be understood that these examples are used to illustrate the present invention and not to limit the scope of the present invention. The implementation conditions used in the examples can be further adjusted according to the conditions of specific manufacturers, and the implementation conditions not indicated are usually the conditions in routine experiments.
[0025] 1. Reagents and materials
[0026] 1. Reagents
[0027] Fetal bovine serum (Hyclone, USA); complete medium: add 100ml of fetal bovine serum, 0.15g of L-glutamine, 10.0ml of 2-mercaptoethanol (5×10 -3 mol / L); liposome 2000 (Invitrogen, USA); penicillin, streptomycin (Shanghai Sangon Biotechnology Co., Ltd); TRIzol (Invitrogen, USA); agarose (LP0028A, Oxoid Ltd, UK); gel Electrophoresis sample solution and ethidium bromide (EtBr) (Shanghai Sangon Biotechnology Co., Ltd.); gel recovery DNA kit an...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com