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Method for extracting polyphenol from Calophyllum Inophyllum L

A technology of sea red fruit and red fruit, applied in the field of polyphenol extraction, to achieve the effect of simple process, easy industrial production, and enhanced antioxidant activity

Inactive Publication Date: 2012-11-07
COFCO GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report about the polyphenols of sea red fruit

Method used

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  • Method for extracting polyphenol from Calophyllum Inophyllum L
  • Method for extracting polyphenol from Calophyllum Inophyllum L

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The sea red fruit was pitted and beaten, 40g of sea red fruit pulp was weighed, and 400 mL of 60% ethanol aqueous solution was added, mixed, and leached in a water bath at 80°C for 3 hours, cooled to room temperature, filtered, and the residue was extracted by repeating the above steps once . The two extracts were combined and concentrated to 1 / 4 of the original volume at 45°C and a vacuum of 0.1 MPa. The concentrated solution was pumped into the balanced NKA-9 macroporous resin column (produced by Beijing Kebao Biotech Co., Ltd.) at a flow rate of 1 mL / min. First rinse the column with 5 times the column volume of distilled water, then use 3 times the column volume of 60% ethanol aqueous solution to elute at a flow rate of 2 mL / min, collect the eluate of 60% ethanol aqueous solution, and depressurize at 45°C and 0.1 MPa concentrate. The concentrated solution was freeze-dried at -60°C and 20 Pa to obtain 0.35 g of pink sea red fruit polyphenol powder, in which the polyp...

Embodiment 2

[0048] The sea red fruit is pitted and beaten, 40g sea red fruit pulp is weighed, and 500 mL of 50% ethanol aqueous solution is added, mixed, and then leached in a 75℃ water bath for 5 hours, cooled to room temperature, filtered, and the residue is extracted once by repeating the above steps . The two extracts were combined, concentrated at 45°C and a vacuum of 0.1 MPa to remove ethanol, and the polyphenol aqueous solution was pumped into the balanced NKA-9 macroporous resin column at a flow rate of 2 mL / min. First rinse the column with 5 times the column volume of distilled water, then use 3 times the column volume of 60% ethanol aqueous solution at a flow rate of 2mL / min, collect the 60% ethanol eluate, concentrate under reduced pressure at 45℃, 0.1MPa, and concentrate The liquid was freeze-dried to obtain 0.33 g of pink sea red fruit polyphenol powder, in which the polyphenol purity was 78% and the FRAP value was 20.03.

Embodiment 3

[0050] The sea red fruit is pitted and beaten, 40g of sea red fruit pulp is weighed, and 600 mL of 50% ethanol aqueous solution is added, mixed, and then leached in a water bath at 85°C for 2 hours, cooled to room temperature, filtered, and the residue is extracted once the above steps . The two extracts were combined, concentrated at 45°C and a vacuum of 0.1 MPa to remove ethanol, and the polyphenol aqueous solution was pumped into the balanced NKA-9 macroporous resin column at a flow rate of 2 mL / min. First rinse the column with 5 times the column volume of distilled water, then use 2 times the column volume of 50% ethanol aqueous solution at a flow rate of 2mL / min, collect the 50% ethanol eluate, concentrate under reduced pressure at 45℃, 0.1MPa, and concentrate The liquid was freeze-dried to obtain 0.30 g of pink sea red fruit polyphenol powder, in which the polyphenol purity was 80% and the FRAP value was 20.51.

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Abstract

The invention provides a method for extracting polyphenol from Calophyllum Inophyllum L, comprising the following steps of: (1) mixing a fruit paste of Calophyllum Inophyllum L with an ethanol aqueous solution, and placing in a water-bath for extraction so as to obtain an extract; (2) filtering the extract, and removing ethanol from the obtained filtrate so as to obtain a condensed polyphenol crude extract; (3) loading the polyphenol crude extract into a well-balanced macroporous adsorption resin column, flushing the column by the use of distilled water, carrying out elution by the use of 50-70 vol. % of an ethanol aqueous solution, and collecting an eluate of the ethanol aqueous solution; (4) removing ethanol from the eluate to obtain a condensed eluate; and (5) drying the condensed eluate to obtain a Calophyllum Inophyllum L polyphenol powder. Purity of the Calophyllum Inophyllum L polyphenol product extracted by the method can reach more than 70%, and the antioxidant activity of the product is obviously enhanced. In addition, the method provided by the invention is simple in technology and is easy for industrial production.

Description

Technical field [0001] The invention relates to a method for extracting polyphenols from sea red fruit. Background technique [0002] Malus micromalus makino (Malus micromalus makino) is the fruit of Rosaceae Pear subfamily Apple genus Catalpa. It is mainly distributed in the loess hilly and gully areas bordering Shanxi, Shaanxi and Mongolia in the middle reaches of the Yellow River, including Hequ, Baode and Pianguan in Shanxi; Fugu and Shenmu; Qingshuihe, Zhungeer Banner, Helinger, Liangcheng and other banner counties in Inner Mongolia. Haihong fruit trees have strong drought resistance, cold resistance, wind resistance, resistance to diseases and insect pests, and have a long fruit-bearing period. They are planted in large numbers in this area and are rich in resources. They are important local economic forests and are also of great significance to local soil and water conservation. There are only 2 patents on Haihongguo. CN1120892 reports a fresh-keeping technology of sea r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P39/06A23L1/30A61K131/00
Inventor 丁庆波程正涛任发政郭慧媛王冶杨海莺
Owner COFCO GROUP
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